Optimized protocol for naive human pluripotent stem cell-derived trophoblast induction

Shingo Io; Yoshiki Iemura; Yasuhiro Takashima

doi:10.1016/j.xpro.2021.100921

Optimized protocol for naive human pluripotent stem cell-derived trophoblast induction

STAR Protoc. 2021 Oct 29;2(4):100921. doi: 10.1016/j.xpro.2021.100921. eCollection 2021 Dec 17.

Authors

Shingo Io^{1

2

3}, Yoshiki Iemura^{1

4

5}, Yasuhiro Takashima¹

Affiliations

¹ Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan.
² Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan.
³ Department of Obstetrics and Gynecology, Kosaka Women's Hospital, Osaka 577-0807, Japan.
⁴ Optional Department of Diagnostic Pathology, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan.
⁵ Japan Society for the Promotion of Science, Tokyo 102-0083, Japan.

Abstract

Human trophoblasts arise from the morula as trophectoderm, which differentiates into cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast after implantation. Here, we present a robust step-by-step protocol to induce trophectoderm (TE) from naive human pluripotent stem cells (PSCs) corresponding to pre-implantation epiblast. Our culture system (TE induction and ACE condition) mimics the entire trophoblast development including the molecular events. For complete details on the use and execution of this protocol, please refer to Io et al. (2021).

Keywords: Cell Biology; Cell Differentiation; Cell culture; Developmental biology; Flow Cytometry/Mass Cytometry; Molecular Biology; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods*
Cell Differentiation / physiology
Culture Media / chemistry
Culture Media / metabolism
Humans
Pluripotent Stem Cells / cytology*
Trophoblasts / cytology*

Substances

Culture Media