Chromatographic profiles and antimicrobial activity of the essential oils obtained from some species and cultivars of the Mentheae tribe (Lamiaceae)

Mariia Shanaida; Nataliia Hudz; Marietta Białoń; Maryna Kryvtsowa; Liudmyla Svydenko; Anna Filipska; Piotr Paweł Wieczorek

doi:10.1016/j.sjbs.2021.06.068

Chromatographic profiles and antimicrobial activity of the essential oils obtained from some species and cultivars of the Mentheae tribe (Lamiaceae)

Saudi J Biol Sci. 2021 Nov;28(11):6145-6152. doi: 10.1016/j.sjbs.2021.06.068. Epub 2021 Jun 26.

Authors

Mariia Shanaida¹, Nataliia Hudz², Marietta Białoń³, Maryna Kryvtsowa⁴, Liudmyla Svydenko⁵, Anna Filipska², Piotr Paweł Wieczorek³

Affiliations

¹ Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Ternopil 4600, Ukraine.
² Department of Drug Technology and Biopharmacy, Danylo Halytsky Lviv National Medical University, Lviv 79010, Ukraine.
³ Department of Analytical Chemistry, University of Opole, Opole 45-052, Poland.
⁴ Department of Genetic, Plant Physiology and Microbiology, Uzhgorod National University, Uzhhorod 88000, Ukraine.
⁵ Sector of Mobilization and Conservation of Plant Resources of the Rice Institute of the NAAS, Plodove, Kherson Region, Kherson 74992, Ukraine.

Abstract

The present study was focused on the chemical composition and antimicrobial activity of the essential oils (EsO) obtained from five Lamiaceae representatives grown in the south of Ukraine. Among them are Salvia sclarea L., Monarda didyma (cultivar 'Cambridge Scarlet'), Thymus pulegioides (cultivar '2/6-07'), Thymus vulgaris (cultivar 'Jalos'), and Thymus serpyllum L. The component analysis of the EsO was carried out by gas chromatography method coupled with mass spectrometry (GC-MS). The antimicrobial properties of the EsO were determined using the agar diffusion test against widespread pathogenic bacterial strains (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Streptococcus pyogenes) and opportunistic yeast Candida albicans. The EsO of Thymus serpyllum and Thymus vulgaris (cultivar 'Jalos') displayed noteworthy antibacterial properties against a wide spectrum of the microorganisms. These antimicrobial properties could be attributed to the high content of aromatic monoterpenoid thymol (52.56% and 47.33%, respectively). The EsO of Salvia sclarea with the dominance of linalyl acetate (45.51%) and linalool (38.98%) as well as Thymus pulegioides (cultivar '2/6-07') containing α-citral (27.10%) and β-citral (17.11%) demonstrated the strongest antimicrobial effects on typical and clinical strains of Staphylococcus aureus with the inhibition zones in the range of 24.0-31.0 mm. The Salvia sclarea EsO demonstrated the most significant effect against clinical strains of Candida albicans. In conclusion, the present study revealed the chemical composition of five Lamiaceae species and cultivars grown in the south of Ukraine and considerable antimicrobial activity of the tested EsO, especially against the typical and clinical strains of Staphylococcus aureus and Candida albicans. The obtained results could be perspective for applying in the pharmaceutical industry and for the conservation of food and cosmetic products.

Keywords: Antimicrobial effect; Cultivar; Essential oil; GC–MS; Monarda didyma; Salvia sclarea; Thymus genus.