Pyrroloquinoline quinone alleviates oxidative damage induced by high glucose in HepG2 cells

Saad Alkahtani; Saud Alarifi; Abdullah A Alkahtane; Gadah Albasher; Mohammed Al-Zharani; Norah M Alhoshani; Norah S Al-Johani; Nada H Aljarba; Md Saquib Hasnain

doi:10.1016/j.sjbs.2021.06.063

Pyrroloquinoline quinone alleviates oxidative damage induced by high glucose in HepG2 cells

Saudi J Biol Sci. 2021 Nov;28(11):6127-6132. doi: 10.1016/j.sjbs.2021.06.063. Epub 2021 Jun 25.

Authors

Saad Alkahtani¹, Saud Alarifi¹, Abdullah A Alkahtane¹, Gadah Albasher¹, Mohammed Al-Zharani², Norah M Alhoshani¹, Norah S Al-Johani¹, Nada H Aljarba³, Md Saquib Hasnain⁴

Affiliations

¹ Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
² Department of Biology, College of Science, Imam Muhammad Ibn Saud Islamic University, Riyadh, Saudi Arabia.
³ Department of Biology, College of Sciences, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
⁴ Department of Pharmacy, Palamau Institute of Pharmacy, Chianki, Daltonganj 822102, Jharkhand, India.

Abstract

Hyperglycemia as a common metabolic disorder in diabetes led to oxidative stress, inflammation and other complications. Natural and manufactured antioxidants alleviates the side effects of diabetes. The purpose of current study is to investigate the effect of pyrroloquinoline quinine (PQQ) as an antioxidant on the content of glucose-induced oxidative stress generation in the cells of the human hepatocellular liver carcinoma (HepG2) by inhibiting advanced glycation end products (AGEs) formation. The HepG2 cells were exposed to high dose (50 mM) of glucose (HG) only and with PQQ (HG + PQQ). Treatment with high dose increased AGEs formation, expression of receptor for advanced glycation endproducts (RAGE), reactive oxygen species ROS production, and oxidative stress markers in treated HepG2 cells. Interestingly, PQQ significantly reduced AGEs formation and (RAGE) expression, ROS formation, and inflammation induced by glucose. In conclusion, PQQ has a potentiail role as an antioxidant to reduce the oxidative damage during hyperglycemia by AGEs inhibition.

Keywords: Cancer; Glucose; Oxidative stress; Pyrroloquinoline quinone.