Graphene oxide-rhodamine nanocomposite for picomolar detection of chromium(III) by fluorimetry and its biofilm inhibition

Krishnasamy Velmurugan; Nanjan Bhuvanesh; Arul Felix Prakash; Velusamy Maheskumar; Bhojan Vidhya; Sevanan Murugan; Raju Suresh Kumar; Abdulrahman I Almansour; Karthikeyan Perumal; Raju Nandhakumar

doi:10.1007/s00604-021-05057-9

Graphene oxide-rhodamine nanocomposite for picomolar detection of chromium(III) by fluorimetry and its biofilm inhibition

Mikrochim Acta. 2021 Nov 9;188(12):414. doi: 10.1007/s00604-021-05057-9.

Affiliations

¹ Fluorensic Materials Lab, Department of Applied Chemistry, Karunya Institute of Technology and Sciences (Declared As Deemed-To-Be University), Karunya Nagar, Coimbatore, 641114, India.
² Department of Nanosciences and Technology, Karunya Institute of Technology and Sciences (Declared As Deemed-To-Be University), Karunya Nagar, Coimbatore, 641114, India.
³ Department of Applied Physics, Karunya Institute of Technology and Sciences (Declared As Deemed-To-Be University), Karunya Nagar, Coimbatore, 641114, India.
⁴ Department of Applied Physics, Karunya Institute of Technology and Sciences (Declared As Deemed-To-Be University), Karunya Nagar, Coimbatore, 641114, India. vidhya@karunya.edu.
⁵ Department of Biotechnology, Karunya Institute of Technology and Sciences (Declared As Deemed-To-Be University), Karunya Nagar, Coimbatore, 641114, India. micromurugans@gmail.com.
⁶ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.
⁷ Department of Chemistry and Biochemistry, The Ohio State University, 151 W. Woodruff Ave, Columbus, OH, 43210, USA.
⁸ Fluorensic Materials Lab, Department of Applied Chemistry, Karunya Institute of Technology and Sciences (Declared As Deemed-To-Be University), Karunya Nagar, Coimbatore, 641114, India. nandhakumar@karunya.edu.

PMID: 34751825
DOI: 10.1007/s00604-021-05057-9

Abstract

Graphene oxide-rhodamine B hydrazide (GO-RhB) nanocomposite was prepared by a simple chemical method and characterized by various spectroscopic and analytical techniques. GO-RhB nanocomposite potentially detects Cr³⁺ ion (excitation/emission = 550 nm/572 nm) via fluorescence turn "on-off" approach. This composite showed high binding affinity (10⁶ M^-1) with Cr³⁺ and a+ limit of detection (LOD) down to picomolar concentration (LOD = 85.6 pM). As far as we know, this is the first report for the sensing of Cr³⁺ ion at picomolar concentration. GO-RhB selectively senses Cr³⁺ ion without any interference of other coexisting metal ions. In addition, this composite exhibited the dynamic nature of quenching in the presence of Cr³⁺ ion, which is confirmed by the Stern-Volmer plot, fluorescence temperature profiles, and decay time experiments. The GO-RhB nanocomposite-based fluorescent probe was successfully applied to the quantitative detection of Cr³⁺ ion in milk sample (linear range = 2 to 10 nM) with better performance than other existing methods. Besides, this GO-RhB composite showed better antibiofilm activity against Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus (MRSA) by using the Congo red agar and tube method.

Keywords: Biofilm; Chromium (III); Fluorescence; Graphene oxide; Nanocomposite; Rhodamine B.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter baumannii / drug effects
Bacterial Adhesion / drug effects
Biofilms / drug effects*
Chromium / chemistry*
Environmental Pollutants / chemistry
Fluorescence
Fluorometry / methods*
Graphite / chemistry*
Methicillin-Resistant Staphylococcus aureus / drug effects
Nanocomposites / chemistry*
Rhodamines / chemistry*

Substances

Environmental Pollutants
Rhodamines
graphene oxide
Chromium
Graphite