Abstract
By analysis of lncRNA expression profiles of macrophages in response to Mycobacterium tuberculosis (Mtb) infection, we identified novel highly expressed transcripts, unique in encompassing a protein coding transcript- Cytidine Monophosphate Kinase 2 (CMPK2) and a previously identified lncRNA- Negative Regulator of Interferon Response (NRIR). While these transcripts (TILT1, 2,3 - TLR4 and Infection induced Long Transcript) are induced by virulent Mtb as well as lipopolysaccharide (LPS) early, lack of/delayed expression in non-viable Mtb/BCG infected cells, respectively, suggest an important role in macrophage responses. The elevated expression by 3 hr in response to fast growing bacteria further emphasizes the importance of these RNAs in the macrophage infection response. Overall, we provide evidence for the presence of multiple transcripts that form a part of the early infection response programme of macrophages.Abbreviations: IFN: Interferon; NRIR: negative regulator of interferon response; CMPK2: cytidine/ uridine monophosphate kinase; LPS: lipopolysaccharide; LAM: Lipoarabinomannan; PIMs: Phosphatidylinositol Mannosides; TILT1, 2,3: TLR4 and Infection induced Long Transcript; TLR4: Toll-like receptor 4; Mtb: Mycobacterium tuberculosis; BCG: Mycobacterium bovis BCG; MDMs: human monocyte derived macrophages.
Keywords:
Non coding transcript; THP1; TLR4; bacterial infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Epistasis, Genetic
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Gene Expression Regulation
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Host-Pathogen Interactions / genetics*
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Host-Pathogen Interactions / immunology
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Humans
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Macrophages / immunology
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Macrophages / metabolism*
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Macrophages / microbiology*
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Mycobacterium tuberculosis*
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RNA, Long Noncoding / genetics
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Signal Transduction
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Toll-Like Receptor 4 / metabolism
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Transcriptome*
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Tuberculosis / genetics*
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Tuberculosis / immunology
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Tuberculosis / microbiology*
Substances
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RNA, Long Noncoding
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TLR4 protein, human
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Toll-Like Receptor 4
Grants and funding
This work was supported by the Council of Scientific and Industrial Research, India [BSC0123]; Council of Scientific and Industrial Research, India [STS0016].