Differentiation of functional astrocytes from human-induced pluripotent stem cells in chemically defined media

Sylvain Perriot; Mathieu Canales; Amandine Mathias; Renaud Du Pasquier

doi:10.1016/j.xpro.2021.100902

Differentiation of functional astrocytes from human-induced pluripotent stem cells in chemically defined media

STAR Protoc. 2021 Oct 20;2(4):100902. doi: 10.1016/j.xpro.2021.100902. eCollection 2021 Dec 17.

Authors

Sylvain Perriot¹, Mathieu Canales¹, Amandine Mathias¹, Renaud Du Pasquier^{1

2}

Affiliations

¹ Laboratory of Neuroimmunology, Neuroscience Research Centre, Department of Clinical Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne, 1015 Lausanne, Switzerland.
² Service of Neurology, Department of Clinical Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne, 1015 Lausanne, Switzerland.

Abstract

This protocol describes how to obtain human astrocytes from human-induced pluripotent stem cells (hiPSCs) in chemically defined media, without the use of fetal bovine serum (FBS). FBS eases the differentiation of astrocytes but also deeply alters their phenotype, as compared with their in vivo counterparts. Our protocol generates hiPSC-derived astrocytes displaying a phenotype and functions similar to human primary astrocytes, including adequate response to inflammation, neurotransmitter uptake, and trophic support to neurons. For complete details on the use and execution of this protocol, please refer to Perriot et al. (2018).

Keywords: Cell Biology; Cell Differentiation; Cell culture; Neuroscience; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Astrocytes / cytology*
Calcium / metabolism
Cell Culture Techniques / methods*
Cell Differentiation / physiology*
Cells, Cultured
Culture Media / chemistry*
Culture Media / metabolism
Glutamic Acid / metabolism
Humans
Induced Pluripotent Stem Cells / cytology*

Substances

Culture Media
Glutamic Acid
Calcium