Structural analysis and anti-cancer activity of low-molecular-weight chondroitin sulfate from hybrid sturgeon cartilage

Ruiyun Wu; Pinglan Li; Yi Wang; Nan Su; Mengyuan Xiao; Xiaojun Li; Nan Shang

doi:10.1016/j.carbpol.2021.118700

Structural analysis and anti-cancer activity of low-molecular-weight chondroitin sulfate from hybrid sturgeon cartilage

Carbohydr Polym. 2022 Jan 1:275:118700. doi: 10.1016/j.carbpol.2021.118700. Epub 2021 Sep 24.

Authors

Ruiyun Wu¹, Pinglan Li², Yi Wang³, Nan Su⁴, Mengyuan Xiao⁵, Xiaojun Li⁶, Nan Shang⁷

Affiliations

¹ Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: wry0814@cau.edu.cn.
² Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: lipinglan@cau.edu.cn.
³ MOE Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China. Electronic address: wangyi671@mail.tsinghua.edu.cn.
⁴ MOE Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
⁵ Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
⁶ Yangzhou Borui Saccharide Biotech Co., Ltd, Jiangsu 225000, China.
⁷ College of Engineering, China Agricultural University, Beijing 100083, China; Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100083, China. Electronic address: nshang@cau.edu.cn.

PMID: 34742426
DOI: 10.1016/j.carbpol.2021.118700

Abstract

Low-molecular-weight chondroitin sulfate (CS) has attracted widespread attention due to its better bioavailability and bioactivity than native CS. In this study, a low-molecular-weight CS (named SCS-F2) was prepared from hybrid sturgeon (Acipenser schrenckii × Huso dauricus) cartilage by enzymatic depolymerization with high in vitro absorption and anti-cancer activity. The structure of SCS-F2 was characterized and the in vivo biodistribution and colorectal cancer prevention effect was investigated. The results revealed that SCS-F2 consisted of 48.84% ΔDi-6S [GlcUAβ1-3GalNAc(6S)], 32.11% ΔDi-4S [GlcUAβ1-3GalNAc(4S)], 16.05% ΔDi-2S,6S [GlcUA(2S)β1-3GalNAc(6S)] and 3.0% ΔDi-0S [GlcUAβ1-3GalNAc]. Animal study showed that the SCS-F2 could be effectively absorbed and delivered to the tumor site and significantly prevented the growth of HT-29 xenograft by inhibiting cell proliferation and inducing apoptosis without showing any negative effect to normal tissues. Therefore, SCS-F2 could be developed as a potential nutraceutical to protect against colorectal cancer.

Keywords: Chondroitin sulfate; Colorectal cancer prevention; Low molecular weight; Structural characterization; Sturgeon cartilage.

MeSH terms

Animals
Antineoplastic Agents / chemistry
Antineoplastic Agents / metabolism
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Cartilage / chemistry*
Cartilage / metabolism
Cell Line
Cell Proliferation / drug effects
Chondroitin Sulfates / chemistry
Chondroitin Sulfates / metabolism
Chondroitin Sulfates / pharmacology*
Drug Screening Assays, Antitumor
Fishes
Humans
Mice
Molecular Weight
Neoplasms, Experimental / drug therapy
Neoplasms, Experimental / pathology

Substances

Antineoplastic Agents
Chondroitin Sulfates