Design and expression of a bispecific antibody against dengue and chikungunya virus in plants

Abstract

Recombinant proteins have a broad range of applications from basic research to pharmaceutical development. Of utmost importance in the production of recombinant proteins is the selection of the best recombinant protein production system, such that high-quality and functional recombinant proteins are produced. Plants can produce a large quantity of recombinant proteins rapidly and economically. Glycoengineering has created "humanized" plant lines that can produce glycoproteins with specific human glycans with a high level of homogeneity on demand. Here, a detailed protocol was provided to produce a large, multisubunit, and complex bispecific antibody that targets two distinct viruses. The successful production of this multiple-subunit protein demonstrated that plants are the optimal system for the production of recombinant proteins of various sizes and complexity, which can be employed for various applications including diagnostics, therapeutics, and vaccines to combat current and future pandemics.

Keywords: Bifunctional antibody; Bispecific antibody; Chikungunya virus; Dengue virus; Plant expression system; Plant-made biologics; Plant-made pharmaceutical.