LDH nanoparticle adjuvant subunit vaccine induces an effective immune response for porcine epidemic diarrhea virus

Danyi Shi; Baochao Fan; Bing Sun; Jinzhu Zhou; Yongxiang Zhao; Rongli Guo; Zengjun Ma; Tao Song; Huiying Fan; Jizong Li; Li Li; Bin Li

doi:10.1016/j.virol.2021.10.010

LDH nanoparticle adjuvant subunit vaccine induces an effective immune response for porcine epidemic diarrhea virus

Virology. 2022 Jan 2:565:58-64. doi: 10.1016/j.virol.2021.10.010. Epub 2021 Nov 2.

Authors

Danyi Shi¹, Baochao Fan², Bing Sun³, Jinzhu Zhou⁴, Yongxiang Zhao⁵, Rongli Guo⁵, Zengjun Ma⁶, Tao Song⁶, Huiying Fan⁷, Jizong Li⁸, Li Li⁸, Bin Li⁹

Affiliations

¹ Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, 210014, Jiangsu, China; College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, 225009, PR China.
² Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, 210014, Jiangsu, China; School of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
³ Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, QLD, 4072, Australia.
⁴ Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, 210014, Jiangsu, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, 225009, PR China; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
⁵ Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, 210014, Jiangsu, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, 225009, PR China.
⁶ College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, China.
⁷ College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
⁸ Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, 210014, Jiangsu, China.
⁹ Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, 210014, Jiangsu, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, 225009, PR China; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China; 5College of Veterinary Medicine, Nanjing Agricultural University, No.1 Wei-gang, Nanjing, 210095, China. Electronic address: libinana@126.com.

PMID: 34739917
DOI: 10.1016/j.virol.2021.10.010

Abstract

Porcine Epidemic Diarrhea (PED) is a highly contagious intestinal disease which mostly caused by Porcine Epidemic Diarrhea Virus (PEDV). The PED has caused huge economic losses to the pig industry all over the world and a valid PEDV vaccine is needed to prevent the infection. In this study, we constructed expression plasmid based on the spike (S) gene of the epidemic PEDV strain. The recombinant eukaryotic S (Se) and prokaryotic S (Sp) subunit proteins were expressed and purified as vaccine antigens. We designed a new subunit vaccine based on S proteins, adjuvanted with layered double hydroxide (LDH). The results indicated that the LDH adjuvanted subunit vaccines induced a better immune effect in terms of antibody level and cellular immune response. In conclusion, this study showed a new design of a PEDV subunit vaccine with nanotechnology and demonstrated the potential for its clinical application.

Keywords: LDH nanoparticle Adjuvant; PEDV; Spike gene; Subunit vaccines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Vaccine / chemistry
Animals
Antibodies, Viral
Coronavirus Infections / immunology*
Coronavirus Infections / prevention & control
Coronavirus Infections / veterinary
Coronavirus Infections / virology
Epidemics
HEK293 Cells
Humans
Hydroxides / chemistry*
Immunity*
Nanoparticles / chemistry*
Nanotechnology / methods
Porcine epidemic diarrhea virus / immunology*
Recombinant Proteins / immunology
Spike Glycoprotein, Coronavirus / immunology*
Swine
Swine Diseases / prevention & control
Swine Diseases / virology
Vaccine Development / methods
Vaccines, Subunit / immunology*

Substances

Adjuvants, Vaccine
Antibodies, Viral
Hydroxides
Recombinant Proteins
Spike Glycoprotein, Coronavirus
Vaccines, Subunit