Study on the mechanism of inhibiting the calcification of anaerobic granular sludge induced by the addition of trace signal molecule (3O-C6-HSL)

Wencai Fu; Meiling Li; Wenhao Dang; Kaili Zhu; Guoning Chen; Jian Zhang; Shuangfei Wang; Yanzhu Guo; Zhiwei Wang

doi:10.1016/j.biortech.2021.126232

Study on the mechanism of inhibiting the calcification of anaerobic granular sludge induced by the addition of trace signal molecule (3O-C6-HSL)

Bioresour Technol. 2022 Jan;344(Pt A):126232. doi: 10.1016/j.biortech.2021.126232. Epub 2021 Oct 28.

Authors

Wencai Fu¹, Meiling Li¹, Wenhao Dang¹, Kaili Zhu¹, Guoning Chen², Jian Zhang¹, Shuangfei Wang¹, Yanzhu Guo³, Zhiwei Wang⁴

Affiliations

¹ Key Laboratory of Clean Pulp & Papermaking and Pollution Control of Guangxi, College of Light Industrial and Food Engineering, Guangxi University, Nanning 530004, PR China.
² Guangxi Bossco Environment Protection Technology Co., Ltd, Nanning 530007, PR China.
³ Key Laboratory of Clean Pulp & Papermaking and Pollution Control of Guangxi, College of Light Industrial and Food Engineering, Guangxi University, Nanning 530004, PR China; Liaoning Key Laboratory of Pulp and Paper Engineering, Dalian Polytechnic University, Dalian 116034, PR China.
⁴ Key Laboratory of Clean Pulp & Papermaking and Pollution Control of Guangxi, College of Light Industrial and Food Engineering, Guangxi University, Nanning 530004, PR China; Guangxi Bossco Environment Protection Technology Co., Ltd, Nanning 530007, PR China. Electronic address: wangzhiwei@gxu.edu.cn.

PMID: 34737162
DOI: 10.1016/j.biortech.2021.126232

Abstract

Microbiota quorum sensing (QS) induced by 3O-C6-HSL (N-(β-ketocaproyl)-DL-homoserine lactone) inhibited the calcification of anaerobic granular sludge (AnGS), and the mechanism of promoting the activity recovery of calcified AnGS was studied in this paper. Through research, it was speculated that 3O-C6-HSL acted on calcified AnGS residual microorganisms to trigger QS. It enriched many functional microorganisms. For example, it promoted the growth of Methanospirillum. The CO₂ could be consumed quickly by Methanospirillum and reduced the calcium carbonate formation. The increase of microbial biomass would promote the activity of sludge. What's more, the pore size and porosity of sludge would increase, so the mass transfer channel will be broadened at same time. All those, could help the calcified AnGS quickly restore the activity and anaerobic system recover normal, which provided a new idea for the emergency rescue of anaerobic system in the future.

Keywords: Calcified anaerobic granular sludge; Microbial community structure; Porosity; Quorum sensing; Signal molecules.

MeSH terms

4-Butyrolactone
Acyl-Butyrolactones
Anaerobiosis
Microbiota*
Quorum Sensing
Sewage*

Substances

Acyl-Butyrolactones
Sewage
4-Butyrolactone