Dynamics of antibiotics and antibiotic resistance genes in four types of kitchen waste composting processes

Abstract

Kitchen waste might be a potential source of antibiotics and antibiotic resistance genes. Composting is recognized as an effective way for kitchen waste disposal. However, the effects of different kitchen waste composting types on the removal of antibiotics and antibiotic resistance genes haven't been systematically studied. In this study, the dynamics of antibiotics and antibiotic resistance genes from kitchen waste of four composting processes were compared. Results showed that although kitchen waste was composted, it remained an underestimated source of antibiotics (25.9-207.3 μg/kg dry weight) and antibiotic resistance genes (10¹²-10¹⁷ copies/kg dry weight). Dynamic composting processes (i.e., dynamic pile composting and mechanical composting) decreased the antibiotic removal efficiency and increased the abundance of some antibiotic resistance genes (5.35-8534.7% enrichment). Partial least-squares path model analysis showed that mobile genetic elements played a dominant role in driving antibiotic resistance genes dynamics. Furthermore, redundancy analysis revealed that temperature, pH, and water content considerably affected the removal of antibiotics and mobile genetic elements. This study provides further insights into exploring the effective strategies in minimizing the risk of antibiotic resistance from kitchen waste via composting process.

Keywords: Antibiotic; Antibiotic resistance gene; Composting; Kitchen waste; Risk analysis.