Generation of two human induced pluripotent stem cell lines from fibroblasts of unrelated Parkinson's patients carrying the G2019S mutation in the LRRK2 gene (LCSBi005, LCSBi006)

Gabriela Novak; Steven Finkbeiner; Gaia Skibinski; Alexander Skupin

doi:10.1016/j.scr.2021.102569

Generation of two human induced pluripotent stem cell lines from fibroblasts of unrelated Parkinson's patients carrying the G2019S mutation in the LRRK2 gene (LCSBi005, LCSBi006)

Stem Cell Res. 2021 Dec:57:102569. doi: 10.1016/j.scr.2021.102569. Epub 2021 Oct 12.

Authors

Gabriela Novak¹, Steven Finkbeiner², Gaia Skibinski², Alexander Skupin³

Affiliations

¹ The Integrative Cell Signaling Group, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-sur-Alzette, Luxembourg; Gladstone Center for Systems and Therapeutics, Gladstone Institutes and Departments of Neurology and Physiology, University of California, San Francisco San Francisco, CA 94158, USA. Electronic address: gabriela.novak@alumni.utoronto.ca.
² Gladstone Center for Systems and Therapeutics, Gladstone Institutes and Departments of Neurology and Physiology, University of California, San Francisco San Francisco, CA 94158, USA.
³ The Integrative Cell Signaling Group, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-sur-Alzette, Luxembourg. Electronic address: alexander.skupin@gmail.com.

PMID: 34736041
DOI: 10.1016/j.scr.2021.102569

Abstract

Mutations in the LRRK2 gene are known to mediate predisposition to Parkinson disease. Fibroblasts heterozygous for the G2019S LRRK2 mutation were obtained from a 53-year-old male patient with disease onset at 34 years (LCSBi005, ND29542), and from a 63-year-old male patient with disease onset at 56 years (LCSBi006, ND34267). Induced pluripotent stem cell (iPSC) clones were generated for each cell line using Sendai virus. The absence of chromosomal defects was confirmed using array comparative genomic hybridization. The cell lines express pluripotency markers and have the ability to differentiate into all three germ layers.