The aim of this research project was to validate the work-related version of the Basic Psychological Need Satisfaction and Frustration Scale (BPNSFS) into the Polish language and culture. Although studies have demonstrated the benefits associated with basic psychological need satisfaction and the costs associated with need frustration at work, the concept of needs has been neglected both in Polish scientific research and in practical organizational studies. The adaptation of the BPNSFS-Work Domain may change this situation and stimulate research in the Polish community. The scale has been validated in a sample consisting of three occupational groups: healthcare workers, education staff and customer service workers (N = 1315, Mage = 43.8). The findings suggest that the Polish scale has robust psychometric features. The CFA analysis proves that the scale has a six-dimensional structure similar to the original scale. These dimensions show satisfactory to high Cronbach's α and McDonalds ω reliability, and high criterion validity is shown by association of the six need dimensions with correlates of both positive (i.e., engagement, job crafting and self-efficacy) and negative aspects of work (i.e., burnout and stress). The structure of the scale is the same in all three occupational groups, although the regression weights and covariances are only partially invariant. The validated version of the BPNSFS-Work Domain can be used in future basic and applied studies in the paradigm of self-determination theory.