Combination of ultrasonic and acidic pretreatments for enhancing biohythane production from tofu processing residue via one-stage anaerobic digestion

Mahmoud M Ali; Ahmed M Mustafa; Ximing Zhang; Xin Zhang; Umar A Danhassaan; Hongjian Lin; Ungyong Choe; Kaiying Wang; Kuichuan Sheng

doi:10.1016/j.biortech.2021.126244

Combination of ultrasonic and acidic pretreatments for enhancing biohythane production from tofu processing residue via one-stage anaerobic digestion

Bioresour Technol. 2022 Jan;344(Pt B):126244. doi: 10.1016/j.biortech.2021.126244. Epub 2021 Oct 31.

Authors

Mahmoud M Ali¹, Ahmed M Mustafa², Ximing Zhang³, Xin Zhang³, Umar A Danhassaan³, Hongjian Lin³, Ungyong Choe⁴, Kaiying Wang³, Kuichuan Sheng⁵

Affiliations

¹ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Biological Engineering Department, Agricultural Engineering Research Institute, Giza, Egypt.
² State Key Laboratory of Pollution Control and Recourses Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Department of Agricultural Engineering, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt.
³ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
⁴ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Faculty of Environmental Science, University of Science, Yusheng Scientist Road, Unjong 13 District, Pyongyang 00850, Democratic People's Republic of Korea.
⁵ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: kcsheng@zju.edu.cn.

PMID: 34732374
DOI: 10.1016/j.biortech.2021.126244

Abstract

Tofu processing residues (TPR) have received more attention as a source of bioenergy. However, their low solubility has hindered biohythane generation. Consequently, the ultrasonic and H₂SO₄ pretreatments were combined and compared for the first time to improve the hydrolysis of organic matter and carbohydrate and increase free amino nitrogen generation from TPR. Besides, the impact of pretreatments on biohythane generation was investigated. Under the optimal conditions of 7.54% substrate level, 8% H₂SO₄ concentration, 80 °C and 50 min, the coincident ultrasonic-H₂SO₄ pretreatment enriched the contents of soluble chemical oxygen demand, reducing sugar, and free amino nitrogen to 49675 mg/L, 26 g/L, and 1721 mg/L, respectively, greater than individual pretreatments. Also, Biohythane yield increased by 4.24-13.61% over control (389.42 ± 23.7 ml/g-VS_fed). Furthermore, hydrogen yield at 42.5 ± 2.08 and 28.1 ± 1.07 ml/g-VS_fed and sulfate removal efficiency at 93 and 92% were significantly improved with ultrasonic-H₂SO₄ and H₂SO₄ pretreatments, respectively, indicating acidogenic and sulfidogenic activity enhancement.

Keywords: Anaerobic digestion; Biohythane; Sulfuric acid pretreatment; Tofu processing residue; Ultrasonic pretreatment.

MeSH terms

Anaerobiosis
Biological Oxygen Demand Analysis
Hydrogen
Methane
Soy Foods*
Ultrasonics*

Substances

Hydrogen
Methane