Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor

Cheryl S Y Yeap; Thanyarat Chaibun; Su Yin Lee; Bin Zhao; Yuan Jan; Chan La-O-Vorakiat; Werasak Surareungchai; Shiping Song; Benchaporn Lertanantawong

doi:10.1039/d1cc05181d

Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor

Chem Commun (Camb). 2021 Nov 16;57(91):12155-12158. doi: 10.1039/d1cc05181d.

Authors

Cheryl S Y Yeap^{1

2

3}, Thanyarat Chaibun^{1

3}, Su Yin Lee⁴, Bin Zhao^{5

6}, Yuan Jan^{5

7}, Chan La-O-Vorakiat³, Werasak Surareungchai^{3

8}, Shiping Song⁵, Benchaporn Lertanantawong^{1

3}

Affiliations

¹ Biosensors Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom, Thailand. benchaporn.ler@mahidol.ac.th.
² Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Studentská 1402/2, 461 17 Liberec, Czech Republic.
³ Pilot Plant and Development Training Institute, Nanoscience and Nanotechnology Research Cluster, King Mongkut's University of Technology Thonburi (KMUTT), Bang Khun Thian Campus, Bangkok 10150, Thailand.
⁴ Centre of Excellence for Omics-Driven Computational Biodiscovery (COMBio) and Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Kedah, 08100, Malaysia.
⁵ Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
⁶ Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
⁷ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
⁸ School of Bioresources & Technology, King Mongkut's University of Technology Thonburi, Thailand.

PMID: 34726213
DOI: 10.1039/d1cc05181d

Abstract

We report a highly sensitive and selective multiplex assay by empowering an electrochemical DNA sensor with isothermal rolling circle amplification. The assay could simultaneously detect and discriminate three common entero-pathogens in a single reaction, with femtomolar sensitivity. It is useful for field- or resource-limited settings.

MeSH terms

Biosensing Techniques*
DNA / genetics*
Electrochemical Techniques*
Nucleic Acid Amplification Techniques*
Salmonella typhi / isolation & purification*
Shigella flexneri / isolation & purification*
Vibrio cholerae / isolation & purification*

Substances

DNA