Design, synthesis, and evaluation of the self-assembled antimicrobial peptides based on the ovalbumin-derived peptide TK913

Ao Tan; Fusheng Xu; Chikako Yokoyama; Shigekazu Yano; Hiroyuki Konno

doi:10.1002/psc.3375

Design, synthesis, and evaluation of the self-assembled antimicrobial peptides based on the ovalbumin-derived peptide TK913

J Pept Sci. 2022 Apr;28(4):e3375. doi: 10.1002/psc.3375. Epub 2021 Nov 1.

Authors

Ao Tan¹, Fusheng Xu¹, Chikako Yokoyama¹, Shigekazu Yano¹, Hiroyuki Konno¹

Affiliation

¹ Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, Yonezawa, Japan.

PMID: 34725889
DOI: 10.1002/psc.3375

Abstract

The preparation, self-assembly, and antimicrobial activity of peptides based on TK913 is described. TK9Z4 incorporating a Pro-Pro motif exhibited self-assembly but no cytotoxicity. However, peptide TKZ3 (obtained by changing the amino acid sequence of TK9Z4) showed morphological changes at different concentrations, potent antimicrobial activity, low cytotoxicity, and trypsin resistance. Accordingly, TKZ3 is proposed as new AMP derived from ovalbumin-derived peptides.

Keywords: Fmoc-SPPS; antimicrobial peptides; proteolytic resistance; secondary structure; self-assembly.

MeSH terms

Amino Acid Sequence
Antimicrobial Peptides*
Ovalbumin / chemistry
Peptides* / chemistry

Substances

Antimicrobial Peptides
Peptides
Ovalbumin