Multi-Omics Analysis of Mammary Metabolic Changes in Dairy Cows Exposed to Hypoxia

Zhiwei Kong; Bin Li; Chuanshe Zhou; Qinghua He; Yuzhong Zheng; Zhiliang Tan

doi:10.3389/fvets.2021.764135

Multi-Omics Analysis of Mammary Metabolic Changes in Dairy Cows Exposed to Hypoxia

Front Vet Sci. 2021 Oct 14:8:764135. doi: 10.3389/fvets.2021.764135. eCollection 2021.

Authors

Zhiwei Kong^{1

2

3}, Bin Li⁴, Chuanshe Zhou⁵, Qinghua He¹, Yuzhong Zheng³, Zhiliang Tan⁵

Affiliations

¹ Department of Food Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, China.
² Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
³ School of Food Engineering and Biotechnology, Hanshan Nornal University, Chaozhou, China.
⁴ State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Institute of Animal Husbandry and Veterinary, Tibet Autonomous Regional Academy of Agricultural Sciences, Lhasa, China.
⁵ CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.

Abstract

Hypoxia exposure can cause a series of physiological and biochemical reactions in the organism and cells. Our previous studies found the milk fat rate increased significantly in hypoxic dairy cows, however, its specific metabolic mechanism is unclear. In this experiment, we explored and verified the mechanism of hypoxia adaptation based on the apparent and omics results of animal experiments and in vitro cell model. The results revealed that hypoxia exposure was associated with the elevation of AGPAT2-mediated glycerophospholipid metabolism. These intracellular metabolic disorders consequently led to the lipid disorders associated with apoptosis. Our findings update the existing understanding of increased adaptability of dairy cows exposure to hypoxia at the metabolic level.

Keywords: dairy cow; hypoxia; lipid metabolism; lipidomics; metabonomics.