SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores

Jee-Eun Lee; Nari Kim; Minkyo Jung; Ji-Young Mun; Joo-Yeon Yoo

doi:10.1080/15548627.2021.1994297

SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores

Autophagy. 2022 Jul;18(7):1613-1628. doi: 10.1080/15548627.2021.1994297. Epub 2021 Oct 31.

Authors

Jee-Eun Lee¹, Nari Kim¹, Minkyo Jung², Ji-Young Mun², Joo-Yeon Yoo¹

Affiliations

¹ Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
² Neural Circuit Research Group, Korea Brain Research Institute, Daegu, Republic of Korea.

Abstract

ATG2: autophagy related 2; BECN1: beclin 1; COPII: coat protein II; DMSO: dimethyl sulfoxide; EBSS: Earle's balanced salt solution; EGFP: enhanced green fluorescent protein; ER: endoplasmic reticulum; ERES: ER exit site(s); GFP: green fluorescent protein; H89: H-89 dihydrochloride hydrate; LAMP1: lysosomal-associated membrane protein 1; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MTORC1: mechanistic target of rapamycin kinase complex 1; NS5A: nonstructural protein 5A; PIK3C3/VPS34: phosphatidylinositol 3-kinase catalytic subunit type 3; PLA: proximity ligation assay; PtdIns3P: phosphatidylionositol-3-phosphate; RB1CC1/FIP200: RB1 inducible coiled-coil 1; RFP: red fluorescent protein; RPS6KB1/S6K: ribosomal protein S6 kinase B1; SBP: streptavidin binding protein; SEC16A: SEC16 homolog A, endoplasmic reticulum export factor; SEC31A: SEC31 homolog A, COPII coat complex component; siRNA: small interfering RNA; Str: streptavidin; ULK1: unc-51-like autophagy activating kinase 1; VSVG: vesicular stomatitis virus glycoprotein; WIPI2: WD repeat domain, phosphoinositide interacting 2; WT: wild type.

Keywords: Constitutive autophagy; SHISA5/SCOTIN; endoplasmic reticulum exit sites; membrane contact; phagophore.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autophagosomes* / metabolism
Autophagy*
Class III Phosphatidylinositol 3-Kinases / metabolism
Endoplasmic Reticulum / metabolism
Golgi Apparatus / metabolism
Streptavidin / metabolism
Vesicular Transport Proteins / metabolism

Substances

Vesicular Transport Proteins
Streptavidin
Class III Phosphatidylinositol 3-Kinases

Grants and funding

This work was supported by the National Research Foundation of Korea [NRF-2017R1A5A1015366] and KBRI basic research program through Korea Brain Research Institute funded by Ministry of Science and ICT [21-BR-01-11].