lncRNA GAS5 suppresses rheumatoid arthritis by inhibiting miR-361-5p and increasing PDK4

Weifeng Zhang; Bing Li; Nannan Xia; Lijuan Zhu; Zhenshan Zhang; Zhijuan Ren; Luyue Zhang; Pengfei Xu; Feilong Meng; Lixin Feng; Lei Yang

doi:10.1016/j.bbrc.2021.10.040

lncRNA GAS5 suppresses rheumatoid arthritis by inhibiting miR-361-5p and increasing PDK4

Biochem Biophys Res Commun. 2021 Oct 19:583:7-13. doi: 10.1016/j.bbrc.2021.10.040. Online ahead of print.

Authors

Affiliations

¹ Department of Rheumatology and Immunology, Zhengzhou Central Hospital, Zhengzhou, 450007, Henan, China.
² The Second Ward of Comprehensive Internal Medicine, Henan No.3 Provincial People's Hospital, Zhengzhou, 450008, Henan, China.
³ Department of Rheumatology and Immunology, Zhengzhou Central Hospital, Zhengzhou, 450007, Henan, China. Electronic address: leiyang562@163.com.

PMID: 34715498
DOI: 10.1016/j.bbrc.2021.10.040

Abstract

Rheumatoid arthritis (RA) is an inflammatory disease that causes hyperplasia of synovial tissue and cartilage destruction. This research was to investigate the effects of lncRNA GAS5/miR-361-5p/PDK4 on rheumatoid arthritis. By qRT-PCR, GAS5 and PDK4 were found to be overexpressed in synovial tissue, fibroblast-like synoviocytes of RA patients and LPS-induced chondrocytes, while the miR-361-5p expression was significantly reduced. GAS5 overexpression resulted in a decrease in the proliferation and Bcl-2 protein expression, and an increase in the Bax protein level. On the contrary, miR-361-5p sponged by GAS5 could accelerate chondrocyte proliferation, inhibit apoptosis. PDK4 targeted by miR-361-5p could inhibit RA, and partially eliminated the effect of miR-361-5p on RA. Our study suggested that GAS5 suppressed RA by competitively adsorbing miR-361-5p to modulate PDK4 expression.

Keywords: Chondrocytes; Fibroblast-like synoviocytes (FLSs); PDK4; Rheumatoid arthritis (RA); lncRNA GAS5; miR-361-5p.