PRMT1 promotes extracellular matrix degradation and apoptosis of chondrocytes in temporomandibular joint osteoarthritis via the AKT/FOXO1 signaling pathway

Qinhao Shen; Yiwen Xiao; Bei Cheng; Zheyi Sun; Yu Hu; Hefeng Yang; Yingwei Luo

doi:10.1016/j.biocel.2021.106112

PRMT1 promotes extracellular matrix degradation and apoptosis of chondrocytes in temporomandibular joint osteoarthritis via the AKT/FOXO1 signaling pathway

Int J Biochem Cell Biol. 2021 Dec:141:106112. doi: 10.1016/j.biocel.2021.106112. Epub 2021 Oct 27.

Authors

Qinhao Shen¹, Yiwen Xiao², Bei Cheng³, Zheyi Sun⁴, Yu Hu⁵, Hefeng Yang⁶, Yingwei Luo⁷

Affiliations

¹ Department of Periodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China; Yunnan Key Laboratory of Stomatology, Kunming 650500, Yunnan Province, China; The First Dental Clinic of the Affiliated Stomatology Hospital of Kunming Medical University, Kunming 650221, Yunnan Province, China.
² Yunnan Key Laboratory of Stomatology, Kunming 650500, Yunnan Province, China; Department of Orthodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China.
³ Department of Periodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China; Yunnan Key Laboratory of Stomatology, Kunming 650500, Yunnan Province, China.
⁴ Yunnan Key Laboratory of Stomatology, Kunming 650500, Yunnan Province, China.
⁵ Department of Orthodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China.
⁶ Yunnan Key Laboratory of Stomatology, Kunming 650500, Yunnan Province, China. Electronic address: yanghefeng2008@163.com.
⁷ Department of Periodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China; The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China. Electronic address: lyw@ydyy.cn.

PMID: 34715362
DOI: 10.1016/j.biocel.2021.106112

Abstract

Temporomandibular joint osteoarthritis (TMJOA) is a chronic degenerative joint disease characterized by extracellular matrix (ECM) degradation and chondrocyte apoptosis. The aim of this study was to investigate the role of PRMT1 in TMJOA pathogenesis and its underlying molecular mechanism. Compared to the control group, PRMT1 was highly expressed in IL-1β-treated chondrocytes and articular cartilage following MIA injection into rat TMJs. Furthermore, knocking down PRMT1 considerably inhibited ECM degradation and apoptosis induced by IL-1β. Mechanistic analyses further revealed that PRMT1 knockdown activated the PI3K/AKT signaling pathway and prevented FOXO1 from translocating to the nucleus. Moreover, an inhibitor of AKT (LY294002) rescued the effect of PRMT1 knockdown on IL-1β-induced ECM degradation and apoptosis, and AMI-1, a selective inhibitor of PRMT1, inhibited PRMT1 expression and reversed the pathological progress of TMJOA. Thus, our findings suggest that PRMT1 plays an essential role in ECM degradation and chondrocyte apoptosis in TMJOA via the AKT/FOXO1 signaling pathway.

Keywords: AKT; Apoptosis; Extracellular matrix degradation; FOXO1; PRMT1; Temporomandibular joint osteoarthritis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chondrocytes*
Male
Osteoarthritis
Phosphatidylinositol 3-Kinases
Proto-Oncogene Proteins c-akt
Rats

Substances

Proto-Oncogene Proteins c-akt