The role of nanoparticles (titanium dioxide, graphene oxide) on the inactivation of co-existing bacteria in the presence and absence of quartz sand

Abstract

The increased mass production and application of engineered nanomaterials (ENMs) have resulted in the release of nanoparticles (NPs) in the environment, raising uncertainties regarding their environmental impacts. This study examines the effect of graphene oxide (GO) and titanium dioxide (TiO₂) NPs on the inactivation of the three model bacteria originated by mammalians including humans: Escherichia (E.) coli, Enterococcus (E.) faecalis, and Staphylococcus (S.) aureus. A series of dynamic batch experiments were conducted at constant room temperature (22 °C) in order to examine the inactivation of co-existing bacteria by NPs, in the presence and absence of quartz sand. The inactivation experimental data were satisfactorily fitted with a pseudo-first order expression with a time dependent rate coefficient. The inactivation of E. coli and S. aureus was shown to increase in the co-presence of GO or TiO₂ NPs and quartz sand comparing with the presence of GO or TiO₂ NPs alone. For E. faecalis, no clear trend was observed. Moreover, quartz sand was shown to affect inactivation of bacteria by GO and TiO₂ NPs. Among the bacteria examined, the highest inactivation rates were observed for S. aureus.

Keywords: Enterococcus faecalis; Escherichia coli; Graphene oxide; Inactivation; Staphylococcus aureus; Titanium dioxide.