[Isolation and characterization of a polyurethane-degrading bacterium]

Sheng Wu Gong Cheng Xue Bao. 2021 Oct 25;37(10):3675-3684. doi: 10.13345/j.cjb.210411.
[Article in Chinese]

Abstract

Biodegradation of polyurethane (PUR) pollutants by microorganisms has received widespread attention currently. Identification of microorganisms capable of efficiently degrading PUR plastics is a key point. In this study, a strain P10 capable of degrading PUR was isolated from the plastic wastes, and identified as a bacterium belonging to the genus of Brevibacillus based on colony morphology and 16S rDNA phylogenetic analysis. Brevibacillus sp. P10 was capable of degrading 71.4% of waterborne polyurethane (Impranil DLN) after 6 days growth in MSM medium with DLN as a sole carbon source. In addition, strain P10 can use commercial PUR foam as the sole carbon source for growth. Brevibacillus sp. P10 can degrade 50 mg PUR foam after 6 days growth in MSM medium supplemented with 5% (V/V) LB after optimization of degradation conditions. This indicates that Brevibacillus sp. P10 has potential to be used in biodegradation of PUR waste.

Keywords: Brevibacillus; biodegradation; plastic pollution; polyurethane.

MeSH terms

  • Bacteria
  • Biodegradation, Environmental*
  • Phylogeny
  • Polyurethanes*

Substances

  • Polyurethanes