Design of nanoemulgel using Argania spinosa microfibrillated cellulose and natural emulsifiers foreseeing melanogenesis enhancement

Meryem Bouhoute; Mitsutoshi Nakajima; Hiroko Isoda

doi:10.1016/j.carbpol.2021.118632

Design of nanoemulgel using Argania spinosa microfibrillated cellulose and natural emulsifiers foreseeing melanogenesis enhancement

Carbohydr Polym. 2021 Nov 15:274:118632. doi: 10.1016/j.carbpol.2021.118632. Epub 2021 Sep 3.

Authors

Meryem Bouhoute¹, Mitsutoshi Nakajima², Hiroko Isoda³

Affiliations

¹ Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-0006, Japan.
² Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-0006, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
³ Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-0006, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan. Electronic address: isoda.hiroko.ga@u.tsukuba.ac.jp.

PMID: 34702455
DOI: 10.1016/j.carbpol.2021.118632

Abstract

Nanotechnology is a route of choice that improves administration and efficacy of bioactive compounds. In this study, nanoemulgels were prepared using microfibrillated cellulose from Argania spinosa shell (AS-MFC) and Argan shell (ASE) or Argan press cake extracts (APC) as natural emulsifiers. Oil-in-water (O/W) nanoemulsions were prepared using different natural emulsifiers or synthetic emulsifiers and presented a nano size (d_3,2 < 140 nm). Following that, the nanoemulsions were incorporated within AS-MFC matrix and rheological properties confirmed a shear thinning behavior. Confocal micrographs of nanoemulgels confirmed the dispersion of nanoemulsions in the AS-MFC network without affecting the nanoemulsions stability. Finally, in vitro bioassay on B16F10 using ASE or APC nanoemulsions was conducted. This study confirmed cell permeation in B16F10 cells of formulated nanoemulsions and the upregulation of melanin content up to 30% more that the untreated cells. This study designed novel MFC nanoemulgel with high potential application in healthcare and cosmetic field.

Keywords: Cell permeation; Melanin; Microfibrillated cellulose; Nanoemulgels; Nanoemulsion; Surface-actives.

MeSH terms

Cellulose / chemistry*
Emulsifying Agents / chemistry*
Particle Size
Plant Extracts / chemistry*
Sapotaceae / metabolism*

Substances

Emulsifying Agents
Plant Extracts
Cellulose