Studying RNP Composition with RIP

Methods Mol Biol. 2022:2404:157-165. doi: 10.1007/978-1-0716-1851-6_8.

Abstract

RNA is never left alone throughout its life cycle. Together with proteins, RNAs form membraneless organelles, called ribonucleoprotein particles (RNPs) where these two types of macromolecules strongly influence each other's functions and destinies. RNA immunoprecipitation is still one of the favorite techniques which allows to simultaneously study both the RNA and protein composition of the RNP complex.

Keywords: Immunoprecipitation; RNA-binding protein; Ribonucleoprotein.

MeSH terms

  • Immunoprecipitation
  • RNA / genetics*

Substances

  • RNA