Protocols for isolation and characterization of mouse placental hemogenic endothelial cells

Guixian Liang; Baofeng Huang; Fengchao Wang; Feng Liu

doi:10.1016/j.xpro.2021.100884

Protocols for isolation and characterization of mouse placental hemogenic endothelial cells

STAR Protoc. 2021 Oct 11;2(4):100884. doi: 10.1016/j.xpro.2021.100884. eCollection 2021 Dec 17.

Authors

Guixian Liang^{1

2

3}, Baofeng Huang^{1

2

3}, Fengchao Wang⁴, Feng Liu^{1

2

3}

Affiliations

¹ State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
² Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.
³ University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ National Institute of Biological Sciences, Beijing 102206, China.

Abstract

The murine mid-gestational placenta has been identified as a hematopoietic site during embryonic development. Here, we describe a protocol for isolation and characterization of the hemogenic endothelial (HE) cells from mouse placenta. We also describe techniques for dissection of placental tissues and for the optimization of tissue digestion and antibody conjugation conditions to identify HE cells via fluorescence-activated cell sorting. For details on the usage and application of this protocol, please refer to Liang et al. (2021).

Keywords: Cell Biology; Cell culture; Developmental biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques
Female
Flow Cytometry / methods*
Hemangioblasts / cytology*
Mice
Placenta / cytology*
Pregnancy