CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration

Changyang Zhou; Xinde Hu; Cheng Tang; Wenjia Liu; Shaoran Wang; Yingsi Zhou; Qimeng Zhao; Qiyu Bo; Linyu Shi; Xiaodong Sun; Haibo Zhou; Hui Yang

doi:10.1093/nsr/nwaa033

CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration

Natl Sci Rev. 2020 May;7(5):835-837. doi: 10.1093/nsr/nwaa033. Epub 2020 Mar 3.

Authors

Changyang Zhou^{1

2}, Xinde Hu^{1

2}, Cheng Tang^{1

2}, Wenjia Liu³, Shaoran Wang^{1

4}, Yingsi Zhou¹, Qimeng Zhao¹, Qiyu Bo³, Linyu Shi^{1

4}, Xiaodong Sun³, Haibo Zhou¹, Hui Yang¹

Affiliations

¹ Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China.
² College of Life Sciences, University of Chinese Academy of Sciences, China.
³ Department of Ophthalmology, Shanghai General Hospital (Shanghai First People's Hospital), Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Fundus Diseases, China.
⁴ Hui-Gene Therapeutics Co., Ltd. China.

Abstract

RNA-targeting CRISPR system Cas13 offers an efficient approach for manipulating RNA transcripts in vitro. In this perspective, we provide a proof-of-concept demonstration that Cas13-mediated Vegfa knockdown in vivo could prevent the development of laser-induced CNV in mouse model of Age-related macular degeneration.