Effects of mechanosensitive ion channel Piezo1 on proliferation and osteogenic differentiation of human dental follicle cells

Yanyan Xing; Binbin Yang; Yun He; Bingqing Xie; Tianqi Zhao; Junliang Chen

doi:10.1016/j.aanat.2021.151847

Effects of mechanosensitive ion channel Piezo1 on proliferation and osteogenic differentiation of human dental follicle cells

Ann Anat. 2022 Jan:239:151847. doi: 10.1016/j.aanat.2021.151847. Epub 2021 Oct 20.

Authors

Yanyan Xing¹, Binbin Yang¹, Yun He², Bingqing Xie¹, Tianqi Zhao¹, Junliang Chen³

Affiliations

¹ Oral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, China.
² Oral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, China; Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China; Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
³ Oral and Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, China; Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, China; Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. Electronic address: cjlhyy@163.com.

PMID: 34687906
DOI: 10.1016/j.aanat.2021.151847

Abstract

Background: To explore the role of the mechanosensitive ion channel Piezo1 in the proliferation and osteogenic differentiation of human dental follicle cells (hDFCs), and its mechanism, so as to provide the basis for the use of hDFCs to achieve bone regeneration.

Methods: hDFCs were obtained from fresh dental follicle tissues by enzymatic digestion, and cell phenotype and multipotential differentiation were identified. Identification of the expression of mechanosensitive ion channel Piezo1 was performed by immunofluorescence and immunohistochemistry. CCK-8 was used to determine the optimal concentration of the Piezo1 agonist, Yoda1. Then, according to the obtained results, Alizarin red staining, RT-PCR quantitative analysis and Western blot were used to further observe the osteogenic differentiation of hDFCs and its probable mechanism via Wnt/β-catenin signalling. The data were analysed by SPSS 22.0 software.

Results: The results of the concentration gradient experiments indicated that 0.5 µM Piezo1 agonist (Yoda1) enhanced the proliferation of hDFCs. Compared with the control group, a considerable number of calcium nodules showed that activating Piezo1 could promote the osteogenic differentiation of hDFCs. The relative mRNA and protein expression of Piezo1, ALP, RUNX2, OCN and BMP2 in the Piezo1 agonist group were higher than that of the control group. Furthermore, the expression of Wnt3a and β-catenin related to the classical osteogenic pathway were significantly up-regulated in the Piezo1 agonist group.

Conclusion: Activating mechanosensitive ion channel Piezo1 with an appropriate concentration of Yoda1 has a positive effect on the proliferation and osteogenic differentiation of hDFCs. This mechanism of promoting osteogenic differentiation may be mediated by the Wnt/β-catenin pathway.

Keywords: Human dental follicle cells; Mechanosensitive ion channel; Osteogenic differentiation; Wnt/β-catenin pathway.

MeSH terms

Cell Differentiation
Cell Proliferation
Cells, Cultured
Dental Sac*
Humans
Ion Channels / genetics
Osteogenesis*

Substances

Ion Channels
PIEZO1 protein, human