Dynamic Metabolic Response, Clotting Times and Peripheral Indices of Central Fatigue in Horse Competing in a 44 Km Endurance Race

Abstract

A deep knowledge on metabolic response of athlete horse to exercise is of paramount importance for the development of optimal training programs to attain equilibrium between good performance and animal welfare. This study investigated the dynamic change of metabolic, clotting times and peripheral indices of central fatigue in horses competing in an official 44 km endurance race. From 6 horses blood samples were collected at rest (T_PRE), within 5 minutes from the end of race (T_PE5), after 30, 60 minutes after the end of race (T_PE30 and T_PE60) to investigate the values of blood lactate, glucose, platelets (PLTs), serum leucine, isoleucine, valine, tryptophan, the ratio between tryptophan and branched-chain amino acids (BCAAs) levels (Try/BCAAs), total proteins, non-esterified fatty acids, plasma thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time and fibrinogen (Fb). Lactate, PLTs, non-esterified fatty acids, BCAAs and Try/BCAAs showed higher values at T_PE5, T_PE30and T_PE60than T_PRE(P < .0001); glucose showed lower values at T_PE5, T_PE30and T_PE60than T_PRE(P < .0001). Higher TT values were found at T_PE5, T_PE30and T_PE60than T_PRE, and at T_PE5than T_PE30 and T_PE60(P < .0001); Fb was higher at T_PE5than T_PRE, T _PE30and T_PE60(P < .0001). This survey confirms that horses performing prolonged physical exercise experienced glycolysis, lipid mobilization and amino acid metabolism to provide substrates as fuel for muscle contraction.

Keywords: Clotting parameters; Endurance race; Fatigue; Horse; Metabolic response.