Sample optimization for saliva 1H-NMR metabolic profiling

Eleonora Quartieri; Emanuela Casali; Elena Ferrari; Benedetta Ghezzi; Mariana Gallo; Alberto Spisni; Marco Meleti; Thelma A Pertinhez

doi:10.1016/j.ab.2021.114412

Sample optimization for saliva ¹H-NMR metabolic profiling

Anal Biochem. 2022 Mar 1:640:114412. doi: 10.1016/j.ab.2021.114412. Epub 2021 Oct 14.

Authors

Eleonora Quartieri¹, Emanuela Casali², Elena Ferrari³, Benedetta Ghezzi⁴, Mariana Gallo², Alberto Spisni², Marco Meleti⁴, Thelma A Pertinhez¹

Affiliations

¹ Department of Medicine and Surgery, University of Parma, Parma, Italy; Transfusion Medicine Unit, Azienda USL-IRCCS di Reggio Emilia, Reggio Emilia, Italy.
² Department of Medicine and Surgery, University of Parma, Parma, Italy.
³ Department of Medicine and Surgery, University of Parma, Parma, Italy. Electronic address: elena.ferrari@unipr.it.
⁴ Department of Medicine and Surgery, University of Parma, Parma, Italy; Centro Universitario di Odontoiatria, University of Parma, Parma, Italy.

PMID: 34656613
DOI: 10.1016/j.ab.2021.114412

Abstract

Nuclear Magnetic Resonance (NMR) based metabolomic analysis of whole saliva has provided potential diagnostic biomarkers for numerous human diseases contributing to a better understanding of their mechanisms. However, a comprehensive interpretation of the significance of metabolites in whole, parotid, and submandibular/sublingual saliva subtypes is still missing. Precision and reproducibility of sample preparation is an essential step. Here, we present a simple and efficient protocol for saliva ¹H-NMR metabolic profiling. This procedure has been specifically designed and optimized for the identification and quantification of low concentration metabolites (as low as 1.1 μM) and is suitable for all the saliva subtypes.

Keywords: (1)H NMR metabolic profile; Parotid saliva; Sample preparation; Submandibular/sublingual saliva; Whole saliva.

MeSH terms

Proton Magnetic Resonance Spectroscopy
Saliva*