Metal-free colorimetric detection of pyrophosphate ions by the peroxidase-like activity of ATP

Jiahui Wen; Na Huang; Zixuan Wei; Danyang Yi; Yijuan Long; Huzhi Zheng

doi:10.1016/j.saa.2021.120479

Metal-free colorimetric detection of pyrophosphate ions by the peroxidase-like activity of ATP

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 15;267(Pt 2):120479. doi: 10.1016/j.saa.2021.120479. Epub 2021 Oct 9.

Authors

Jiahui Wen¹, Na Huang¹, Zixuan Wei¹, Danyang Yi¹, Yijuan Long¹, Huzhi Zheng²

Affiliations

¹ Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China.
² Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China. Electronic address: zhenghz@swu.edu.cn.

PMID: 34655979
DOI: 10.1016/j.saa.2021.120479

Abstract

Pyrophosphate (P₂O₇^4-, PPi) plays a vital role in ecological environment. Its elevated levels in water bodies can lead to eutrophication. Hence, its detection is extremely significant. Whereas most of the existing methods for the actual detection of PPi may cause environmental pollution or suffer from operational complexity. In this study, we introduced a sensitive and selective method for detecting PPi based on the fact that PPi can inhibit the peroxidase-like activity of adenosine 5'-triphosphate (ATP). This strategy not only eliminated the complexity of material preparation (ATP is commercialized), but also addressed the general need for metal ions in detecting PPi. The dynamic range of PPi detection was 1.0-200 μM and the detection limit was 74 nM. In addition, this strategy had been successfully applied to the determination of PPi in tap water and lake water. This work extends the application of natural biological small molecule ATP in the analysis and provides an innovative thought for the metal-free detection of PPi.

Keywords: ATP; Colorimetric method; Inhibition; Metal-free; Peroxidase-like activity; Pyrophosphate.

MeSH terms

Adenosine Triphosphate
Colorimetry*
Diphosphates*
Metals
Peroxidases

Substances

Diphosphates
Metals
Adenosine Triphosphate
Peroxidases