PCR-based next-generation West Nile virus sequencing protocols

Styliani Pappa; Serafeim C Chaintoutis; Chrysostomos I Dovas; Anna Papa

doi:10.1016/j.mcp.2021.101774

PCR-based next-generation West Nile virus sequencing protocols

Mol Cell Probes. 2021 Dec:60:101774. doi: 10.1016/j.mcp.2021.101774. Epub 2021 Oct 13.

Authors

Styliani Pappa¹, Serafeim C Chaintoutis², Chrysostomos I Dovas², Anna Papa³

Affiliations

¹ National Reference Centre for Arboviruses, Department of Microbiology, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece.
² Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece.
³ National Reference Centre for Arboviruses, Department of Microbiology, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece. Electronic address: annap@auth.gr.

PMID: 34653595
DOI: 10.1016/j.mcp.2021.101774

Abstract

The epidemiology of West Nile virus (WNV) is unpredictable and changing. Availability of whole genome sequences enables the detailed molecular epidemiology studies and the evaluation and design of diagnostic tools. In the present study we provide two PCR-based protocols which can be applied directly on biological samples from hosts infected by WNV strains belonging to lineage 1 or lineage 2. It was shown that the protocols worked successfully even on samples with relatively low viral load.

Keywords: Next generation sequencing; West nile virus; Whole genome.

MeSH terms

High-Throughput Nucleotide Sequencing
Humans
Phylogeny
Polymerase Chain Reaction
West Nile Fever*
West Nile virus* / genetics