c-Jun N-terminal kinase (JNK) in Procambarus clarkii: Molecular characterization and involvement in oxidative stress-induced apoptosis during molting cycle

Hongyan Tian; Wang Yuning; Wenping Yang; Fei Liu; Yebing Yu; Shengjie Ren; Aimin Wang; Dingdong Zhang

doi:10.1016/j.cbpb.2021.110676

c-Jun N-terminal kinase (JNK) in Procambarus clarkii: Molecular characterization and involvement in oxidative stress-induced apoptosis during molting cycle

Comp Biochem Physiol B Biochem Mol Biol. 2022 Jan:257:110676. doi: 10.1016/j.cbpb.2021.110676. Epub 2021 Oct 11.

Authors

Hongyan Tian¹, Wang Yuning², Wenping Yang², Fei Liu², Yebing Yu², Shengjie Ren², Aimin Wang³, Dingdong Zhang⁴

Affiliations

¹ Department of Marine Science and Technology, School of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, PR China. Electronic address: tianhyy@outlook.com.
² Department of Marine Science and Technology, School of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, PR China.
³ Department of Marine Science and Technology, School of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, PR China. Electronic address: blueseawam@ycit.edu.cn.
⁴ Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.

PMID: 34648929
DOI: 10.1016/j.cbpb.2021.110676

Abstract

The present study was conducted to characterize the full-length cDNA of c-Jun N-terminal kinase (JNK) in Procambarus clarkii (Pcjnk) and evaluate its potential function under different molt cycle. The full-length cDNA of Pcjnk covered 2937 bp with an open reading frame of 1320 bp, encoding 439 amino acids. A typical conserved TPY motif (118Thr-Pro-120Tyr) was found in Pcjnk. Quantitative real-time PCR (qRT-PCR) analysis revealed a constitutive expression of Pcjnk in the tested tissue, with the highest expression occurring in the hepatopancreas. Additionally, the present study initially revealed that relative mRNA expression of Pcjnk and apoptosis level were significantly higher in the premolt stage (D1/D2 and D3/D4 stage) as compared to other molt stages. In contrast to the levels of superoxide dismutase (SOD) and malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GPX) level decreased significantly from the intermolt stage (C stage) to the premolt stage (D1/D2 and D3/D4 stage), then increased from the premolt stage to the postmolt stage (A and B stage). The results obtained in the present study indicated that molt could cause apoptosis induced by oxidative stress through the activation of JNK in Procambarus clarkii.

Keywords: Gene cloning; JNK; Molt; Oxidative stress; Procambarus clarkii.

MeSH terms

Animals
Apoptosis
Astacoidea*
JNK Mitogen-Activated Protein Kinases
Molting*
Oxidative Stress

Substances

JNK Mitogen-Activated Protein Kinases