Evaluating the biocompatibility and biological performance of FePt-decorated nano-epoxy nanoparticles in mesenchymal stem cells

Ruey-Hwang Chou; Meng-Yin Yang; Yi-Ping Yang; Chun-An Yeh; Wei-Yi Lai; Tzu-Wei Lin; Yuan-Chi Teng; Shu-Chen Hsieh; Mei-Lang Kung; Hsien-Hsu Hsieh; Huey-Shan Hung

doi:10.1097/JCMA.0000000000000627

Evaluating the biocompatibility and biological performance of FePt-decorated nano-epoxy nanoparticles in mesenchymal stem cells

J Chin Med Assoc. 2021 Dec 1;84(12):1109-1119. doi: 10.1097/JCMA.0000000000000627.

Authors

Ruey-Hwang Chou^{1

2

3}, Meng-Yin Yang^{4

5

6}, Yi-Ping Yang⁷, Chun-An Yeh¹, Wei-Yi Lai⁷, Tzu-Wei Lin⁷, Yuan-Chi Teng⁷, Shu-Chen Hsieh⁸, Mei-Lang Kung⁹, Hsien-Hsu Hsieh¹⁰, Huey-Shan Hung^{1

11}

Affiliations

¹ Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, ROC.
² Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan, ROC.
³ Department of Medical Laboratory and Biotechnology, Asia University, Taichung, Taiwan, ROC.
⁴ Neurological Institute Head of Department of Neurosurgery Taichung Veterans General Hospital, Taichung, Taiwan, ROC.
⁵ College of Nursing, Central Taiwan University of Science and Technology, Taichung, Taiwan, ROC.
⁶ College of Medicine, National chung Hsing University, Taichung, Taiwan, ROC.
⁷ Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
⁸ Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC.
⁹ Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, ROC.
¹⁰ Blood Bank, Taichung Veterans General Hospital, Taichung, Taiwan, ROC.
¹¹ Translational Medicine Research, China Medical University Hospital, Taichung, Taiwan, ROC.

PMID: 34643620
DOI: 10.1097/JCMA.0000000000000627

Abstract

Background: Nanoparticles have wide potential applications in biolabeling, bioimaging, and cell tracking. Development of dual functional nanoparticles increases the versatility.

Methods: We combined the fluorescent property of nano-epoxy (N-Epo) and the magnetic characteristic of FePt to fabricate the FePt-decorated N-Epo (N-Epo-FePt). The size in diameter of N-Epo-FePt (177.38 ± 39.25 nm) was bigger than N-Epo (2.28 ± 1.01 nm), both could be absorbed into mesenchymal stem cells (MSCs) via clathrin-mediated endocytosis and have multiple fluorescent properties (blue, green, and red).

Results: N-Epo-FePt prevented N-Epo-induced platelet activation, CD68+-macrophage differentiation in blood, and intracellular ROS generation in MSCs. The induction of apoptosis and the inhibitory effects of N-Epo-FePt on cell migration, MMP-9 activity, and secretion of SDF-1α were less than that of N-Epo in MSCs.

Conclusion: N-Epo-FePt was more biocompatible without altering biological performance than N-Epo in MSCs. These results suggest that N-Epo-FePt nanoparticle can be used for fluorescence labeling of MSCs and is potential to apply to bioimaging and cell tracking of MSCs in vivo by magnetic resonance imaging or computed tomography.

MeSH terms

Cell Line, Tumor
Humans
Materials Testing*
Mesenchymal Stem Cells*
Microscopy, Fluorescence
Nanoparticles* / chemistry
Tomography, X-Ray Computed