The Inhibition Effect of Photo-Cross-Linking between Probes in Photo-Induced Double Duplex Invasion DNA

Chembiochem. 2021 Dec 10;22(24):3402-3405. doi: 10.1002/cbic.202100430. Epub 2021 Oct 22.

Abstract

Double duplex invasion (DDI) DNA is a useful antigene method that inhibits expression of genomic DNA. We succeeded in performing photoinduced-DDI (pDDI) using ultrafast photo-cross-linking. 5-Cyanouracil (CN U) has been used in pDDI to inhibit photo-cross-linking between probes, but its importance has not been clarified. Therefore, in this study, we evaluated the effect of spacer (S) and d-spacer (dS) that exhibit photo-cross-linking ability similar to that of CN U. CN U exhibited the highest pDDI efficiency, and S, dS, and T were not very different. The photo-cross-linking inhibitory effect was better with S and dS than with thymidine (T). Conversely, the thermal stability was significantly lower with S and dS than with T. The results suggest that the pDDI efficiency is determined by the balance between the photo-cross-linking inhibitory effect and the thermal stability, which is the introduction efficiency for double-stranded DNA. Therefore, CN U, which has a photo-cross-linking inhibitory effect and a high Tm value, showed the highest inhibitory efficiency.

Keywords: DNA photo-cross-linking; antigenes; double duplex invasion DNA; nucleic acids; photochemistry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbazoles / chemistry
  • Carbazoles / pharmacology*
  • Cross-Linking Reagents / chemistry
  • Cross-Linking Reagents / pharmacology*
  • DNA / drug effects*
  • Molecular Structure
  • Photosensitizing Agents / chemistry
  • Photosensitizing Agents / pharmacology*

Substances

  • Carbazoles
  • Cross-Linking Reagents
  • Photosensitizing Agents
  • DNA