Analysis of Flavonoid Metabolites in Watercress (Nasturtium officinale R. Br.) and the Non-Heading Chinese Cabbage (Brassica rapa ssp. chinensis cv. Aijiaohuang) Using UHPLC-ESI-MS/MS

Molecules. 2021 Sep 26;26(19):5825. doi: 10.3390/molecules26195825.

Abstract

Flavonoids from plants play an important role in our diet. Watercress is a special plant that is rich in flavonoids. In this study, four important watercress varieties were compared with non-heading Chinese cabbage by ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS). A total of 132 flavonoid metabolites (including 8 anthocyanins, 2 dihydroflavone, 3 dihydroflavonol, 1 flavanols, 22 flavones, 11 flavonoid carbonosides, 82 flavonols, and 3 isoflavones) were detected. Flavonoid metabolites varied widely in different samples. Both the non-heading Chinese cabbage and the variety of watercress from Guangdong, China, had their own unique metabolites. This work is helpful to better understand flavonoid metabolites between the non-heading Chinese cabbage and the other four watercress varieties, and to provide a reliable reference value for further research.

Keywords: Brassica rapa ssp. chinensis cv. Aijiaohuang; Nasturtium officinale R. Br.; OPLS-DA; PCA; UHPLC-ESI-MS/MS; flavonoid metabolites.

MeSH terms

  • Brassica rapa / metabolism*
  • China
  • Chromatography, High Pressure Liquid / methods*
  • Flavonoids / analysis*
  • Metabolome*
  • Nasturtium / metabolism*
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • Flavonoids