Sulfur transformation and bacterial community dynamics in both desulfurization-denitrification biofilm and suspended activated sludge

Abstract

Types of microbial aggregates have essential effects on bacterial communities' characteristics, thus affecting the pollutants removal. An up-flow biofilm reactor was used to study the different performances of S^2-/NO₂^- removal and functional genes in suspended sludge and biofilms. The metabolic pathways of sulfurous and nitrogenous pollutants in the desulfurization-denitrification process were proposed. The results showed that S⁰ formation dominated the reactor with a high S^2- concentration. Autotrophic Sulfurovum responsible for S^2-/S⁰ oxidation was the only dominant bacteria in suspended sludge. Heterotrophic Desulfocapsa responsible for SO₄^2- reduction coexisted with Sulfurovum and dominated in biofilms. S^2- oxidation to S⁰ was catalyzed via fccA/B and sqr genes in suspended sludge. S₃^2-/S⁰ oxidation to SO₄^2- was catalyzed via dsrA/B gene in biofilms. SO₄^2- and NO₂^- were removed via the dissimilatory sulfate reduction and denitrification pathway, respectively. This work provides a fundamental and practical basis for optimizing suspended sludge/biofilm systems for S^2-/NO₂^- removal.

Keywords: Biofilm; Functional genes; Metabolic pathway; Suspended sludge; Trisulfide.