Cell imaging with multi-color DNA framework probes

Mo Xie; Linjie Guo; Shu Xing; Shuting Cao; Ziwei Zhao; Kaiyi Liang; Jiang Li; Shihua Luo; Yu Zhang; Lianhui Wang

doi:10.1039/d1cc04305f

Cell imaging with multi-color DNA framework probes

Chem Commun (Camb). 2021 Oct 28;57(86):11318-11321. doi: 10.1039/d1cc04305f.

Authors

Mo Xie¹, Linjie Guo^{2

3}, Shu Xing⁴, Shuting Cao³, Ziwei Zhao^{1

5}, Kaiyi Liang⁶, Jiang Li^{2

3}, Shihua Luo⁷, Yu Zhang^{2

3}, Lianhui Wang¹

Affiliations

¹ State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China. iamlhwang@njupt.edu.cn.
² Bioimaging Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, The Interdisciplinary Research Center, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China. zhangyu@sari.ac.cn.
³ Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
⁴ Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, P. R. China.
⁵ Shanghai Frontier Innovation Research Institute Co., Ltd., Shanghai 201108, China.
⁶ Radiology Department of Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Key Laboratory of Shanghai Municipal Health Commission for Smart Image, Shanghai 201800, China.
⁷ Department of Traumatology, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China. jqab@163.com.

PMID: 34635891
DOI: 10.1039/d1cc04305f

Abstract

Utilizing the programmability of the fractal DNA frameworks, multi-color probes were constructed by arranging fluorescent molecules and nucleic acid aptamers on the structure. Multiplexed cell imaging and classification was realized through pattern recognition.

MeSH terms

Aptamers, Nucleotide / chemistry*
Cell Membrane Permeability
DNA Probes / chemistry*
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Microscopy, Fluorescence
Nanostructures / chemistry
Nucleic Acids / chemistry*
Optical Imaging

Substances

Aptamers, Nucleotide
DNA Probes
Fluorescent Dyes
Nucleic Acids