Examining the effect of manganese on physiological processes: Invertebrate models

Abstract

Manganese (Mn²⁺ as MnSO₄ &/or MnCl₂) is a common and essential element for maintaining life in plants and animals and is found in soil, fresh waters and marine waters; however, over exposure is toxic to organisms. MnSO₄ is added to soil for agricultural purposes and people are exposed to Mn²⁺ in the mining industry. Hypermanganesemia in mammals is associated with neurological issues mimicking Parkinson's disease (PD) and appears to target dopaminergic neural circuits. However, it also seems that hypermanganesemia can affect many aspects of health besides dopaminergic synapses. We examined the effect on development, behavior, survival, cardiac function, and glutamatergic synaptic transmission in the Drosophila melanogaster. In addition, we examined the effect of Mn²⁺ on a sensory proprioceptive organ and nerve conduction in a marine crustacean and synaptic transmission at glutamatergic neuromuscular junctions of freshwater crayfish. A dose-response effect of higher Mn²⁺ retards development, survival and cardiac function in larval Drosophila and survival in larvae and adults. MnSO₄ as well as MnCl₂ blocks stretch activated responses in primary proprioceptive neurons in a dose-response manner. Mn²⁺ blocks glutamatergic synaptic transmission in Drosophila as well as crayfish via presynaptic action. This study is relevant in demonstrating the effects of Mn²⁺ on various physiological functions in order to learn more about acute and long-term consequences Mn²⁺ exposure.

Keywords: Cardiac; Crab; Crayfish; Drosophila; Manganese; Neuromuscular junction; Sensory; Survival.