Dynamics of microbial community and their effects on membrane fouling in an anoxic-oxic gravity-driven membrane bioreactor under varying solid retention time: A pilot-scale study

Anjan Deb; Khum Gurung; Jannatul Rumky; Mika Sillanpää; Mika Mänttäri; Mari Kallioinen

doi:10.1016/j.scitotenv.2021.150878

Dynamics of microbial community and their effects on membrane fouling in an anoxic-oxic gravity-driven membrane bioreactor under varying solid retention time: A pilot-scale study

Sci Total Environ. 2022 Feb 10;807(Pt 2):150878. doi: 10.1016/j.scitotenv.2021.150878. Epub 2021 Oct 8.

Authors

Anjan Deb¹, Khum Gurung², Jannatul Rumky², Mika Sillanpää³, Mika Mänttäri², Mari Kallioinen²

Affiliations

¹ Department of Separation Science, School of Engineering Science, Lappeenranta-Lahti University of Technology (LUT University), Sammonkatu 12, 50130 Mikkeli, Finland; Department of Chemistry, University of Helsinki, P.O. Box 55 (A.I. Virtasen aukio 1), 00014 Helsinki, Finland. Electronic address: anjan.deb@helsinki.fi.
² Department of Separation Science, School of Engineering Science, Lappeenranta-Lahti University of Technology (LUT University), Sammonkatu 12, 50130 Mikkeli, Finland.
³ Department of Chemical Engineering, School of Mining, Metallurgy and Chemical Engineering, University of Johannesburg, P. O. Box 17011, Doornfontein 2028, South Africa; School of Chemical and Metallurgical Engineering, University of the Witwatersrand, 2050 Johannesburg, South Africa; Chemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; School of Resources and Environment, University of Electronic Science and Technology of China (UESTC), NO. 2006, Xiyuan Ave., West High-Tech Zone, Chengdu, Sichuan 611731, PR China; Faculty of Science and Technology, School of Applied Physics, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia; School of Chemistry, Shoolini University, Solan, Himachal Pradesh 173229, India.

PMID: 34627895
DOI: 10.1016/j.scitotenv.2021.150878

Abstract

Membrane fouling in a membrane bioreactor (MBR) is highly influenced by the characteristics of the influent, the mixed liquor microbial community and the operational parameters, all of which are environment specific. Therefore, we studied the dynamics of microbial community during the treatment of real municipal wastewater in a pilotscale anoxic-oxic (A/O) MBR equipped with a gravity-driven membrane filtration system. The MBR was operated at three different solid retention times (SRTs): 25, 40 and 10 days for a total period of 180 days in Nordic environmental conditions. Analysis of microbial community dynamics revealed a high diversity of microbial species at SRT of 40 days, whereas SRT of 25 days was superior with microbial richness. Production of soluble microbial products (SMP) and extracellular polymeric substances (EPS) was found to be intensely connected with the SRT and food to microorganism (F/M) ratio. Relatively longer operational period with the lowest rate of membrane fouling was observed at SRT of 25 days, which was resulted from the superior microbial community, lowest production of SMP and loosely bound EPS as well as the lower filtration resistance of larger sludge flocs. Abundance of quorum quenching (QQ) bacteria and granular floc forming bacterial genera at SRT of 25 days provided relatively lower membrane fouling tendency and larger floc formation, respectively. On the other hand, substantial amount of various surface colonizing and EPS producing bacteria was found at SRT of 10 days, which promoted more rapid membrane fouling compared with the fouling rate seen at other tested SRTs. To sum up, this research provides a realistic insight into the impact of SRT on microbial community dynamics and resulting characteristics of mixed liquor, floc size distribution and membrane fouling for improved MBR operation.

Keywords: Floc size distribution; Membrane bioreactor; Membrane fouling; Microbial community dynamics; Solid retention time.

MeSH terms

Bioreactors*
Microbiota*