Chitosan and κ-carrageenan-derived nitrogen and sulfur co-doped carbon dots "on-off-on" fluorescent probe for sequential detection of Fe3+ and ascorbic acid

Abstract

This study develops a high sensitive and selective "on-off-on" fluorescent probe for sequential detection of iron ion (Fe³⁺) and ascorbic acid (AA) based on nitrogen and sulfur co-doped carbon dots (N, S-CDs), which were synthesized by using chitosan and κ-carrageenan as raw materials through one-step hydrothermal protocol. The synthesized N,S-CDs possess particularly high quantum yield (QY = 59.31%), excellent stability and excitation dependent behavior, showing great potential for practical applications. Furthermore, N,S-CDs provided high selectivity and strong anti-interference to Fe³⁺ due to its fluorescence quenching performance, revealing a wide linear concentration range from 1 to 100 μM for the detection of Fe³⁺ ion with an extremely low limit of detection of 57 nM, and presented reliable and accurate results in actual sample detection of Fe³⁺. The overall fluorescence quenching mechanism of N,S-CDs with Fe³⁺ was due to the formation of N,S-CDs/Fe³⁺ initiated to the aggregation and electron transfer of N,S-CDs, resulting in the static quenching of fluorescence. More interestingly, AA could reduce Fe³⁺ to Fe²⁺ and efficaciously recover the quenched fluorescence of N,S-CDs/Fe³⁺. N,S-CDs/Fe³⁺ as "turn-on" fluorescent probe was further applied for detecting AA in a linear range of 0.5-90 μM with a detection limit of 38 nM.

Keywords: Ascorbic acid; Chitosan; Fe(3+) ion detection; N,S-CDs; κ-Carrageenan.