A disposable aptasensor based on a gold-plated coplanar electrode array for on-site and real-time determination of Cu2

Haochen Qi; Xiaofan Huang; Jayne Wu; Jian Zhang; Fei Wang; Hao Qu; Lei Zheng

doi:10.1016/j.aca.2021.338991

A disposable aptasensor based on a gold-plated coplanar electrode array for on-site and real-time determination of Cu²

Anal Chim Acta. 2021 Oct 23:1183:338991. doi: 10.1016/j.aca.2021.338991. Epub 2021 Aug 25.

Authors

Haochen Qi¹, Xiaofan Huang², Jayne Wu³, Jian Zhang⁴, Fei Wang⁵, Hao Qu⁶, Lei Zheng⁷

Affiliations

¹ College of Electrical and Electronic Engineering, Wenzhou University, Wenzhou, 325035, China; School of Electronic Science and Applied Physics, Hefei University of Technology, Hefei, 230009, China.
² School of Electronic Science and Applied Physics, Hefei University of Technology, Hefei, 230009, China.
³ Department of Electrical Engineering and Computer Science, The University of Tennessee, Knoxville, TN, 37996, USA. Electronic address: jaynewu@utk.edu.
⁴ College of Electrical and Electronic Engineering, Wenzhou University, Wenzhou, 325035, China; School of Electronic Science and Applied Physics, Hefei University of Technology, Hefei, 230009, China. Electronic address: zhangjian@hfut.edu.cn.
⁵ Beijing Smartchip Microelectronics Technology Company Limited, Beijing, 102200, China.
⁶ School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China.
⁷ School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China. Electronic address: lei.zheng@aliyun.com.

PMID: 34627507
DOI: 10.1016/j.aca.2021.338991

Abstract

Copper ion (Cu²⁺) is an important cofactor for many enzymes in human body. Either excessive or deficient Cu²⁺ in the body may cause serious dysfunctions and diseases. So sensitive determination of Cu²⁺ in environmental samples is of more significance for evaluation and control of Cu²⁺ intake. Based on a low-cost gold-plated coplanar electrode array, a disposable aptasensor is developed with an ultra-sensitive indicator of interfacial capacitance. Modified with a specially isolated DNA aptamer for Cu²⁺, this sensor achieves a high selectivity of 1207: 1 against non-target ions. To realize real-time response, alternating-current electrothermal effect is integrated into the capacitance measuring process to efficiently enrich the trace Cu²⁺. This sensor reaches a limit of detection of 2.97 fM, with a linear range from 5.0 fM to 50 pM. The response time is only 15 s, which can meet the real-time detection requirement. On-site test of practical samples is also realized using the disposable sensor combined with a handheld inductance/capacitance/resistance meter. This sensor with its portable test system provides a cost-efficient solution for on-site, real-time and sensitive detection of Cu²⁺, showing great application value in environment monitoring.

Keywords: Aptasensor; Capacitive sensing; Cu(2+) determination; On-site and real-time detection.

MeSH terms

Aptamers, Nucleotide*
Electrochemical Techniques
Electrodes
Gold*
Humans
Limit of Detection

Substances

Aptamers, Nucleotide
Gold