Objective: To investigate the expressions of microRNAs in peripheral blood of patients with primary immune thrombocytopenia(ITP) and its correlation with the imbalance of Th1/Th2 cells.
Methods: Thirty patients with ITP (ITP group) and 15 healthy people (control group) were enrolled．Real-time polymerase chain reaction (RT-PCR) was used to detect the expressions of six miRNAs (miR-107,miR-205-5p,miR-138-5p,miR-326,miR-1827,miR-185-5p) and Th1-specific transcription factor T-bet mRNA and Th2-specific transcription factor GATA-3 mRNA in the peripheral blood of the two groups. Th1 and Th2 cells were detected by flow cytometry. The expressions of Th1-cytokines TNF-α and IFN-γ and Th2-cytokines IL-4 and IL-10 were detected by AimPlex multiple immunoassays for Flow. The expression difference of miRNAs, mRNA, Th1, Th2 cells and cytokines of the two groups were compared, and the correlations of miRNAs to mRNA, Th1, Th2 cells and cytokines were analyzed in ITP group.
Results: The expressions of miRNAs（miR-107, miR-205-5p, miR-138-5p, miR-326, miR-1827, miR-185-5p）and Th2-specific transcription factor GATA-3 mRNA of the patients in ITP group were significantly decreased (P<0.05) as compared with those in control, while the expressions of Th1 cells and Th1-specific transcription factor T-bet mRNA and Th1-cytokines TNF-α were significantly increased (P<0.05), also for the ratios of T-bet mRNA/GATA-3 mRNA and Th1/Th2 cells were significantly increased (P<0.05). The relative expressions of miR-107, miR-205-5p, miR-138-5p in ITP patients were negatively correlated with Th2 cells (r=-0.411, r=-0.593, r=-0.403,P<0.05) and the relative expression of miR-1827 was negatively correlated with TNF-α (r=-0.390).
Conclusion: The relative expressions of the six miRNAs in peripheral blood of patients with ITP are significantly decreased, which result in the increasing ratio of T-bet mRNA/GATA-3 mRNA, then lead to the imbalance of Th1/Th2.
题目: 原发性免疫性血小板减少症患者外周血microRNAs的表达及与Th1/Th2失衡的关系.
目的: 探讨原发性免疫性血小板减少症（ITP）患者外周血micoRNAs的表达量与Th1/Th2失衡的关系.
方法: 纳入30例ITP患者(ITP组)及15例健康人(对照组)，采用实时定量 PCR(RT-PCR)法检测2组外周血中6种miRNA（miR-107、miR-205-5p、miR-138-5p、miR-326、miR-1827、miR-185-5p）和Th1特异性转录因子T-bet、Th2特异性转录因子GATA-3的表达水平，采用流式细胞术检测Th1、Th2细胞，AimPlex流式细胞术高通量技术检测Th1细胞因子TNF-α、IFN-γ以及Th2细胞因子IL-4、IL-10含量。比较2组间miRNAs、mRNA、Th1、Th2细胞及其分泌的主要细胞因子的差异,分析ITP患者miRNAs与Th1、Th2细胞及其分泌的主要细胞因子的相关性.
结果: 与对照组相比，ITP组miRNA（miR-107、miR-205-5p、miR-138-5p、miR-326、miR-1827、miR-185-5p）和Th2细胞特异性转录因子GATA-3的表达水平均显著降低(P<0.05)；Th1细胞、Th1细胞特异性转录因子T-bet及Th1细胞因子TNF-α表达水平均显著升高(P<0.05)，T-bet/GATA-3和Th1/Th2比值均显著升高（P<0.05）。ITP患者miR-107、miR-205-5p、miR-138-5p相对表达量与Th2细胞均呈负相关（P＜0.05，r=-0.411； r=-0.593； r=-0.403）；miR-1827相对表达量与TNF-α呈负相关（r=-0.390）.
结论: ITP患者外周血6种miRNAs表达水平显著降低，导致T-bet mRNA/GATA-3 mRNA比值升高，Th1/Th2失衡.