The mitochondrial genome of Eurycantha calcarata Lucas, 1869 (Phasmatodea: Lonchodinae) and its phylogeny

Ke-Ke Xu; Qing-Ping Chen; Jia-Yin Guan; Zi-Yi Zhang; Kenneth B Storey; Dan-Na Yu; Jia-Yong Zhang

doi:10.1080/23802359.2021.1964403

The mitochondrial genome of Eurycantha calcarata Lucas, 1869 (Phasmatodea: Lonchodinae) and its phylogeny

Mitochondrial DNA B Resour. 2021 Sep 30;6(11):3109-3111. doi: 10.1080/23802359.2021.1964403. eCollection 2021.

Authors

Ke-Ke Xu¹, Qing-Ping Chen¹, Jia-Yin Guan¹, Zi-Yi Zhang¹, Kenneth B Storey², Dan-Na Yu^{1

3}, Jia-Yong Zhang^{1

3}

Affiliations

¹ College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, PR China.
² Department of Biology, Carleton University, Ottawa, Canada.
³ Key Lab of Wildlife Biotechnology, Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua, PR China.

Abstract

The Lonchodinae (Phasmatodea: Phasmatidae) is rich in insect species with more than 330 species of 40 genera. The phylogenetic relationships within Lonchodinae have been under debate. We successfully sequenced the complete mitogenome of Eurycantha calcarata Lucas, 1869 (Phasmatodea: Lonchodinae) with a length of 16,280 bp, which had the same genes and gene arrangements as those of various published papers on stick insects. The whole mitogenome and control region of E. calcarata had a high AT content of 78.2 and 85.9%, respectively. All PCGs used ATN as the start codon, and most PCGs used TAA/TAG as the stop codons excluding COX2 (T), COX3 (TA), and ND5 (TA). To discuss the phylogeny of Lonchodinae, we reconstructed the phylogenetic relationships of 27 species of Phasmatodea including E. calcarata and two species of Embioptera used as outgroups. In BI and ML trees, the monophyly of Lonchodinae and Necrosciinae was well supported, whereas the monophyly of Clitumninae was not recovered. These results indicated that Lonchodinae was a sister clade to Phylliinae and E. calcarata was a sister clade to Phraortes genus.

Keywords: Lonchodinae; mitogenome; phylogeny.

Grants and funding

This work was supported by the Natural Science Foundation of Zhejiang Province [LY18C040004], the College Students’ Innovation and Entrepreneurship Project of China [201910345030, 202010345026, and 202110345014], and the College Students’ Innovation and Entrepreneurship Project of Zhejiang Province [202010345R128]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.