Background: Oxidative stress status in different cancer types was investigated before, but not studied in gastric intestinal metaplasia to the best of our knowledge. Purpose of this study is to examine whether there is a difference between oxidative stress status in patients with intestinal metaplasia (IM) compared to individuals without IM, we compared the serum levels of disulfide (SS), total thiol (TT) and native thiol (NT).
Methods: This was a prospective, non-randomized casecontrol study including 67 patients with histopathologically confirmed IM and 60 individuals demographically matched in terms of age, gender, BMI, smoking status, and chronic diseases as control group.
Results: The mean NT, TT and NT to TT (NT/TT) ratios were statistically significantly higher in IM group compared to controls ((351.71 ± 81.9 mol/L vs. 271.82 ± 54.13 mol/L, p=0.000), (391.5 ± 92.69 mol/L vs. 308.59 ± 55.53 mol/L, p=0.000) and (0.89 ± 0.6 vs. 0.87 ± 0.29, p=0.022), respectively). The mean SS to TT (SS/TT) ratio was significantly lower in IM group than control group (0.050 ± 0.31 vs. 0.060 ± 0.014, P=0.022). Median SS and mean SS/NT ratio was similar in both groups (16.3 (3.3-78) vs. 18.3 (10-32.7), p=0.271 and 0.055 ± 0.041 vs. 0.070 ± 0.019, p=0.068, respectively). In ROC analysis, cut off value of SS/NT for IM was found 0.062, in regression analysis, SS/NT <0.062 was found as an independently prognostic marker for IM (OR, 2.38; 95%CI: 1.168-4.865, P=0.017).
Conclusions: SS/NT ratio lower than 0.062 was found as an independently prognostic marker for IM. This ratio could help to distinguish which patients should be followed closely for gastric cancer.
Uvod: Status oksidativnog stresa kod različitih vrsta karcinoma istražen je ranije, ali nije proučavan u intestinalnoj metaplaziji želuca prema našem najboljem znanju. Svrha ove studije je da ispita da li postoji razlika između statusa oksidativnog stresa kod pacijenata sa intestinalnom metaplazijom (IM) u poređenju sa osobama bez IM, tako što su upoređeni nivoi serumskog disulfida (SS), ukupnog tiola (TT) i nativnog tiola (NT).
Metode: Ovo je bila prospektivna, nerandomizirana studija slučaja i kontrole, koja je obuhvatala 67 pacijenata sa histo patološki potvrđenim IM i 60 osoba demografski podudarnih u pogledu starosti, pola, BMI-a, statusa pušenja i hroničnih bolesti kao kontrolna grupa.
Rezultati: Srednji odnosi NT, TT i NT prema TT (NT/TT) bili su statistički značajno veći u IM grupi u poređenju sa kontrolama (351,71 ± 81,9 mol/L naspram 271,82 ± 54,13 mol/L, p=0,000), (391,5 ± 92,69 mol/L na - suprot 308,59 ± 55,53 mol/L, p=0,000) i (0,89 ± 0,6 naspram 0,87 ± 0,29, p=0,022), respektivno. Srednji odnos SS prema TT (SS/TT) bio je značajno niži u IM grupi od kontrolne grupe (0,050 ± 0,31 naspram 0,060 ± 0,014, P=0,022). Medijana SS i srednji odnos SS/NT bili su slični u obe grupe 16,3 (3,3-78) naspram 18,3 (10-32,7), p=0,271 i 0,055 ± 0,041 naspram 0,070 ± 0,019, p=0,068, respektivno). U ROC analizi utvrđena je granična vrednost SS/NT za IM 0,062, u regresionoj analizi SS/NT <0,062 kao nezavisno prognostički marker za IM (OR, 2,38; 95% CI: 1,168-4,865, P=0,017).
Zaključak: SS/NT odnos niži od 0,062 pronađen je kao nezavisno prognostički marker za IM. Ovaj odnos bi mogao da pomogne da se dijagnostikuju pacijenti kod kojih treba pažljivo pratiti rak želuca.
Keywords: disulphide; intestinal metaplasia; oxidative stress; tiol.
2021 Nilay Danis, Aysegul Ertinmaz Ozkan, Fatih Karatas, Cagri Cakici, Turkan Yigitbasi, Nurhayat Ozkan Sevencan, Burcak Kayhan, published by CEON/CEES.