An adaptive optics module for deep tissue multiphoton imaging in vivo

Cristina Rodríguez; Anderson Chen; José A Rivera; Manuel A Mohr; Yajie Liang; Ryan G Natan; Wenzhi Sun; Daniel E Milkie; Thomas G Bifano; Xiaoke Chen; Na Ji

doi:10.1038/s41592-021-01279-0

An adaptive optics module for deep tissue multiphoton imaging in vivo

Nat Methods. 2021 Oct;18(10):1259-1264. doi: 10.1038/s41592-021-01279-0. Epub 2021 Oct 4.

Authors

Cristina Rodríguez^#^{1

2

3}, Anderson Chen^#^{2

4}, José A Rivera¹, Manuel A Mohr⁵, Yajie Liang^{2

6}, Ryan G Natan^{2

3}, Wenzhi Sun^{2

7

8}, Daniel E Milkie², Thomas G Bifano^{9

4}, Xiaoke Chen⁵, Na Ji^{10

11

12

13

14}

Affiliations

¹ Department of Physics, University of California, Berkeley, CA, USA.
² Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
³ Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
⁴ Bio Optical and Acoustic Spectroscopy Laboratory, Neurophotonics Center, Boston University, Boston, MA, USA.
⁵ Department of Biology, Stanford University, Stanford, CA, USA.
⁶ Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.
⁷ School of Basic Medical Sciences, Capital Medical University, Beijing, China.
⁸ Chinese Institute for Brain Research, Beijing, China.
⁹ Department of Mechanical Engineering, Photonics Center, Boston University, Boston, MA, USA.
¹⁰ Department of Physics, University of California, Berkeley, CA, USA. jina@berkeley.edu.
¹¹ Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. jina@berkeley.edu.
¹² Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. jina@berkeley.edu.
¹³ Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA. jina@berkeley.edu.
¹⁴ Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. jina@berkeley.edu.

^# Contributed equally.

Abstract

Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins
Embryo, Nonmammalian
Female
Green Fluorescent Proteins
Luminescent Proteins
Male
Mice
Neuroimaging / methods*
Optics and Photonics / methods*
Zebrafish

Substances

Bacterial Proteins
Luminescent Proteins
yellow fluorescent protein, Bacteria
Green Fluorescent Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding