A near-infrared fluorescent probe based on the hemicyanine skeleton for the detection of hydrogen peroxide in vivo

Abstract

As a member of the reactive oxygen species, hydrogen peroxide (H₂O₂) plays critical roles in oxidative stress and cell signaling. Intracellular abnormal levels of H₂O₂ production are closely related to many diseases. Therefore, the real-time monitoring of H₂O₂ in the cells is important. In this work, we designed a novel fluorescent probe (Mito-H₂O₂) for the specific detection of H₂O₂ based on the hemicyanine skeleton, with bright near-infrared fluorescence emission. Mito-H₂O₂ displayed fast response, excellent water-solubility and great fluorescence intensity enhancement after the addition of H₂O₂. Furthermore, Mito-H₂O₂ has been successfully applied to image both of the exogenous and endogenous H₂O₂ in cells and mice with negligible cytotoxity.

Keywords: Bioimaging; Fluorescent probe; Hydrogen peroxide; Mitochondria; Near-infrared.