SNX20AR/MiRNA-301a-3p/SNX20 Axis Associated With Cell Proliferation and Immune Infiltration in Lung Adenocarcinoma

Yixiao Yuan; Xiulin Jiang; Lin Tang; Juan Wang; Qianqian Liu; Xiaolan Zou; Lincan Duan

doi:10.3389/fmolb.2021.744363

SNX20AR/MiRNA-301a-3p/SNX20 Axis Associated With Cell Proliferation and Immune Infiltration in Lung Adenocarcinoma

Front Mol Biosci. 2021 Sep 17:8:744363. doi: 10.3389/fmolb.2021.744363. eCollection 2021.

Authors

Yixiao Yuan^{1

2}, Xiulin Jiang³, Lin Tang^{1

2}, Juan Wang^{1

2}, Qianqian Liu^{1

2}, Xiaolan Zou², Lincan Duan^{1

2}

Affiliations

¹ Department of Graduate School of Kunming Medical University, Kunming, China.
² Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
³ Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing, China.

Abstract

Lung cancer is the most common tumor with severe morbidity and high mortality. Increasing evidence has demonstrated that SNX20 plays crucial roles in the progression of human cancer. However, the functions and mechanism of SNX20 in LUAD are still barely known. Here, we employ the TCGA, GEO and CCLE databases to examine the expression of SNX20 in human varies cancer, the results shown that SNX20 is down-regulated in lung Adenocarcinoma, SNX20 level was significantly positive correlated with poor prognosis and lung cancer immune cell infiltration. We found that over-expression of SNX20 significantly restrain NSCLC cell proliferation and migration. Subsequently, we discover a network regulating SNX20 in LUAD, further study found that the decreased of the SNX20 likely caused by DNA hypermethylation. Furthermore, we identified that SNX20AR/miRNA-301a-3p mediated decreased of SNX20 correlated with lung cancer progression and cancer immune infiltration in LUAD. Our findings suggested that ncRNAs play a crucial role in the regulatory network of SNX20. Collectively, our findings demonstrate the suppressor roles of the SNX20AR/miRNA-301a-3p/SNX20 axis in Lung Adenocarcinoma, represent that SNX20 have the potential of as an effective therapeutic target in future.

Keywords: LUAD; SNX20; cell proliferation; immune infiltration; lncRNA; miRNA-301a-3p.