Sensitive colorimetric aptasensor based on stimuli-responsive metal-organic framework nano-container and trivalent DNAzyme for zearalenone determination in food samples

Yuhan Sun; Yan Lv; Shuo Qi; Yin Zhang; Zhouping Wang

doi:10.1016/j.foodchem.2021.131145

Sensitive colorimetric aptasensor based on stimuli-responsive metal-organic framework nano-container and trivalent DNAzyme for zearalenone determination in food samples

Food Chem. 2022 Mar 1:371:131145. doi: 10.1016/j.foodchem.2021.131145. Epub 2021 Sep 15.

Authors

Yuhan Sun¹, Yan Lv¹, Shuo Qi¹, Yin Zhang², Zhouping Wang³

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
² Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China. Electronic address: wangzp@jiangnan.edu.cn.

PMID: 34600366
DOI: 10.1016/j.foodchem.2021.131145

Abstract

Zearalenone (ZEN) poses a serious threat to human and animal health. The development of sensitive determination methods for ZEN is of great significance for ensuring the quality and safety of food. Herein, based on a stimuli-responsive aptamer-functionalized metal-organic framework (MOF) nano-container and trivalent DNA peroxidase mimicking enzyme (DNAzyme), an efficient aptasensor was constructed initially for the colorimetric determination of ZEN. The proposed aptasensor only required simple operations but exhibited outstanding specificity, reproducibility, storage stability and reusability simultaneously. Under the optimal conditions, there was a good linear relationship between the changed absorbance and logarithm concentration of ZEN within 0.01-100 ng mL^-1, and the limit of detection (LOD) could reach 0.36 pg mL^-1. Moreover, the proposed aptasensor was reliable in quantifying ZEN in spiked food samples. The current bioassay provides a promising scheme for constructing stable, specific and rapid colorimetric platforms with potential applications in the fields of food safety.

Keywords: Colorimetric aptasensor; DNAzyme; Nano-container; Stimuli-responsive; Zearalenone.

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques*
Colorimetry
DNA, Catalytic*
Humans
Limit of Detection
Metal-Organic Frameworks*
Reproducibility of Results
Zearalenone* / analysis

Substances

Aptamers, Nucleotide
DNA, Catalytic
Metal-Organic Frameworks
Zearalenone