rGO based immunosensor amplified using MWCNT and CNF nanocomposite as analytical tool for CA125 detection

Maalavika S Iyer; Fu-Ming Wang; Ramana Ramya Jayapalan; Subha Veeramani; Ilangovan Rajangam

doi:10.1016/j.ab.2021.114393

rGO based immunosensor amplified using MWCNT and CNF nanocomposite as analytical tool for CA125 detection

Anal Biochem. 2021 Dec 1:634:114393. doi: 10.1016/j.ab.2021.114393. Epub 2021 Sep 28.

Authors

Maalavika S Iyer¹, Fu-Ming Wang², Ramana Ramya Jayapalan¹, Subha Veeramani¹, Ilangovan Rajangam³

Affiliations

¹ National Centre for Nanoscience and Nanotechnology, University of Madras, Chennai, 600 025, Tamil Nadu, India.
² Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, 106, Taiwan, ROC.
³ National Centre for Nanoscience and Nanotechnology, University of Madras, Chennai, 600 025, Tamil Nadu, India. Electronic address: rajangamilangovan@gmail.com.

PMID: 34597616
DOI: 10.1016/j.ab.2021.114393

Abstract

The electrochemical performance of dual layer immunosensor has been studied by employing reduced Graphene oxide (rGO) and its nanocomposites with Carbon Nanofibers (CNFs) and Carbon Nanotubes (CNTs) as supporting matrix for the detection of CA125. The immunosensor determination was based on the formation of antibody - antigen immunocomplex, a decrement in the current response was observed in accordance with the concentration of antigen. Better performance exhibited by rGO/CNF in terms of linearity (99%) and sensitivity 0.65 μA (μg mL^-1)^-1 can be attributed to its conductivity and surface area. The nanocomposite are employed in the detection of CA125 with linear working range of 10-32 × 10^-4 μg mL^-1, the limit of detection is found to be 0.28 pg mL^-1 rGO nanocomposite with CNT (rGO/CNT) is studied as transducer material. rGO/CNT exhibited better linearity when compared to rGO due to its good conductivity. Thus, graphene nanocomposite transducer materials have vital application in detection of oncomarkers.

Keywords: Carbon nanofiber; Carbon–carbon composites; Immunosensor; Physical properties; Thionine nanohybrids.

MeSH terms

Biosensing Techniques / methods*
CA-125 Antigen / analysis*
Carbon / chemistry
Electric Conductivity
Electrochemical Techniques
Gold / chemistry
Graphite / chemistry*
Humans
Immunoassay / methods
Limit of Detection
Metal Nanoparticles / chemistry
Nanocomposites / chemistry*
Nanofibers / chemistry
Nanotubes, Carbon / chemistry*
Photoelectron Spectroscopy / methods

Substances

CA-125 Antigen
Nanotubes, Carbon
graphene oxide
Carbon
Gold
Graphite