The objective of the current study was to compare the mycochemical composition and biological activities of two extracts (aqueous extracts and ground materials prepared with the QUENCHER approach) of three wild Basidiomy-cetes, Agaricus vaporarius, Lactarius deliciosus, and Lycoperdon pyriforme, collected from a region located in the north of Tunisia. Mycochemical analysis in terms of total phenolic compounds, flavonoids, flavanols, proanthocyanidins, and tannin contents were determined. In addition, the antioxidant activities of the mushrooms were evaluated using three assays (2,2-di-phenyl-1-picrylhydrazyl free radical scavenging, ferric reducing antioxidant power assay, and metal chelating assay). Their antimicrobial activities were assessed against seven bacterial species. The obtained data revealed the presence of significant differences between secondary metabolites and the biological activities of the different tested extracts. Extracts of Basidiomy-cetes prepared with the QUENCHER approach had higher total phenolic content and antimicrobial activity against the evaluated strains. Antioxidant activity showed that the water extract had the greatest activity. The water extracts were analyzed with high-performance liquid chromatography, and the results showed that these three wild macromycetes contain a wide variety of antioxidant compounds in addition to many other bioactive compounds of nutritional and therapeutic interest.